A single double-strand break system reveals repair dynamics and mechanisms in heterochromatin and euchromatin [electronic resource].
- Bethesda, Md. : National Institutes of Health (U.S.), 2016. and Oak Ridge, Tenn. : Distributed by the Office of Scientific and Technical Information, U.S. Dept. of Energy
- Physical Description:
- pages 1,645-1,657 : digital, PDF file
- Additional Creators:
- Lawrence Berkeley National Laboratory, National Institutes of Health (U.S.), United States. Department of Energy, and United States. Department of Energy. Office of Scientific and Technical Information
- Repair of DNA double-strand breaks (DSBs) must be properly orchestrated in diverse chromatin regions to maintain genome stability. The choice between two main DSB repair pathways, nonhomologous end-joining (NHEJ) and homologous recombination (HR), is regulated by the cell cycle as well as chromatin context.Pericentromeric heterochromatin forms a distinct nuclear domain that is enriched for repetitive DNA sequences that pose significant challenges for genome stability. Heterochromatic DSBs display specialized temporal and spatial dynamics that differ from euchromatic DSBs. Although HR is thought to be the main pathway used to repair heterochromatic DSBs, direct tests of this hypothesis are lacking. Here, we developed an in vivo single DSB system for both heterochromatic and euchromatic loci in Drosophila melanogaster Live imaging of single DSBs in larval imaginal discs recapitulates the spatio-temporal dynamics observed for irradiation (IR)-induced breaks in cell culture. Importantly, live imaging and sequence analysis of repair products reveal that DSBs in euchromatin and heterochromatin are repaired with similar kinetics, employ both NHEJ and HR, and can use homologous chromosomes as an HR template. This direct analysis reveals important insights into heterochromatin DSB repair in animal tissues and provides a foundation for further explorations of repair mechanisms in different chromatin domains.
- Published through SciTech Connect., 07/15/2016., "ark:/13030/qt04c739ct", Genes and Development 30 14 ISSN 0890-9369 AM, and Aniek Janssen; Gregory A. Breuer; Eva K. Brinkman; Annelot I. van der Meulen; Sean V. Borden; Bas van Steensel; Ranjit S. Bindra; Jeannine R. LaRocque; Gary H. Karpen.
- Funding Information:
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