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Detection of Lipomannan in Cattle Infected with Bovine Tuberculosis [electronic resource].

Published:
Washington, D.C. : United States. Dept. of Energy, 2017.
Oak Ridge, Tenn. : Distributed by the Office of Scientific and Technical Information, U.S. Dept. of Energy
Physical Description:
457-460 : digital, PDF file
Additional Creators:
Los Alamos National Laboratory, United States. Department of Energy, United States. Department of Agriculture, and United States. Department of Energy. Office of Scientific and Technical Information
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Summary:
Early and rapid detection of bovine tuberculosis (bTB) is critical to controlling the spread of this disease in cattle and other animals. Here in this study, we demonstrate the development of an immunoassay for the direct detection of the bovine bTB biomarker, lipomannan (LM) in serum using a waveguide-based optical biosensor. We apply an ultra-sensitive detection strategy developed by our team, termed lipoprotein capture, that exploits the pull-down of high-density lipoprotein (HDL) nanodiscs from cattle blood that allows for the recovery and detection of associated LM. We also profile the change in the expression of these TB biomarkers as a function of time from a small set of samples collected from studies of bovine TB-infected cattle. Lastly, we demonstrate for the first time the direct detection of bovine LM in serum, and clearly show that the biomarker is expressed in detectable concentrations during the entire course of the infection.
Report Numbers:
E 1.99:la-ur-17-21083
la-ur-17-21083
Subject(s):
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Note:
Published through SciTech Connect.
04/10/2017.
"la-ur-17-21083"
Analytical Sciences 33 4 ISSN 0910-6340 AM
Dung M. Vu; Rama M. Sakamuri; W. Ray Waters; Basil I. Swanson; Harshini Mukundan.
New Mexico Small Business Assistance (NMSBA)
Funding Information:
AC52-06NA25396
View MARC record | catkey: 23497903