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Dynamics and heterogeneity of a fate determinant during transition towards cell differentiation [electronic resource].

Published
Washington, D.C. : United States. National Nuclear Security Administration, 2015.
Oak Ridge, Tenn. : Distributed by the Office of Scientific and Technical Information, U.S. Dept. of Energy
Physical Description
23 pages : digital, PDF file
Additional Creators
United States. National Nuclear Security Administration and United States. Department of Energy. Office of Scientific and Technical Information
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Summary
Yan is an ETS-domain transcription factor responsible for maintaining Drosophila eye cells in a multipotent state. Yan is at the core of a regulatory network that determines the time and place in which cells transit from multipotency to one of several differentiated lineages. Using a fluorescent reporter for Yan expression, we observed a biphasic distribution of Yan in multipotent cells, with a rapid inductive phase and slow decay phase. Transitions to various differentiated states occurred over the course of this dynamic process, suggesting that Yan expression level does not strongly determine cell potential. Consistent with this conclusion, perturbing Yan expression by varying gene dosage had no effect on cell fate transitions. However, we observed that as cells transited to differentiation, Yan expression became highly heterogeneous and this heterogeneity was transient. Signals received via the EGF Receptor were necessary for the transience in Yan noise since genetic loss caused sustained noise. As a result, since these signals are essential for eye cells to differentiate, we suggest that dynamic heterogeneity of Yan is a necessary element of the transition process, and cell states are stabilized through noise reduction.
Report Numbers
E 1.99:1257261
Subject(s)
Note
Published through SciTech Connect.
11/19/2015.
eLife 4 ISSN 2050-084X AM
Nicolas Pelaez; Arnau Gavalda-Miralles; Bao Wang; Heliodoro Tejedor Navarro; Herman Gudjonson; Ilaria Rebay; Aaron R. Dinner; Aggelos K. Katsaggelos; Luís A. N. Amaral; Richard W. Carthew.
Northwestern Univ., Evanston, IL (United States)
Funding Information
NA0002520
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