Actions for Three-dimensional structural dynamics of DNA origami Bennett linkages using individual-particle electron tomography [electronic resource].

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Three-dimensional structural dynamics of DNA origami Bennett linkages using individual-particle electron tomography [electronic resource].

Published
Washington, D.C. : United States. Dept. of Energy. Office of Basic Energy Sciences, 2018.
Oak Ridge, Tenn. : Distributed by the Office of Scientific and Technical Information, U.S. Dept. of Energy
Physical Description
Article numbers: 592 : digital, PDF file
Additional Creators
Lawrence Berkeley National Laboratory, United States. Department of Energy. Office of Basic Energy Sciences, National Science Foundation (U.S.), National Institutes of Health (U.S.), and United States. Department of Energy. Office of Scientific and Technical Information
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Summary
Scaffolded DNA origami has proven to be a powerful and efficient technique to fabricate functional nanomachines by programming the folding of a single-stranded DNA template strand into three-dimensional (3D) nanostructures, designed to be precisely motion-controlled. Although two-dimensional (2D) imaging of DNA nanomachines using transmission electron microscopy and atomic force microscopy suggested these nanomachines are dynamic in 3D, geometric analysis based on 2D imaging was insufficient to uncover the exact motion in 3D. In this paper, we use the individual-particle electron tomography method and reconstruct 129 density maps from 129 individual DNA origami Bennett linkage mechanisms at ~6-14 nm resolution. The statistical analyses of these conformations lead to understanding the 3D structural dynamics of Bennett linkage mechanisms. Moreover, our effort provides experimental verification of a theoretical kinematics model of DNA origami, which can be used as feedback to improve the design and control of motion via optimized DNA sequences and routing.
Report Numbers
E 1.99:1433119
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Note
Published through SciTech Connect.
02/09/2018.
"ark:/13030/qt6b7353nt"
Nature Communications 9 1 ISSN 2041-1723 AM
Dongsheng Lei; Alexander E. Marras; Jianfang Liu; Chao-Min Huang; Lifeng Zhou; Carlos E. Castro; Hai-Jun Su; Gang Ren.
Univ. of California, San Francisco, CA (United States)
Funding Information
AC02-05CH11231
DMR-1344290
AC02-05CH11231
R01HL115153
R01GM104427
CMMI-1536862
View MARC record | catkey: 23498191