Investigation of the chemical interface in the soybean–aphid and rice–bacteria interactions using MALDI-mass spectrometry imaging [electronic resource].

Published: Washington, D.C. : United States. Dept. of Energy, 2015.
Oak Ridge, Tenn. : Distributed by the Office of Scientific and Technical Information, U.S. Dept. of Energy
Physical Description: pages 5,294-5,301 : digital, PDF file
Additional Creators: Ames Laboratory, United States. Department of Energy, and United States. Department of Energy. Office of Scientific and Technical Information

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Summary

Mass spectrometry imaging (MSI) is an emerging technology for high-resolution plant biology. It has been utilized to study plant–pest interactions, but limited to the surface interfaces. Here we expand the technology to explore the chemical interactions occurring inside the plant tissues. Two sample preparation methods, imprinting and fracturing, were developed and applied, for the first time, to visualize internal metabolites of leaves in matrix-assisted laser desorption ionization (MALDI)-MSI. This is also the first time nanoparticle-based ionization was implemented to ionize diterpenoid phytochemicals that were difficult to analyze with traditional organic matrices. The interactions between rice–bacterium and soybean–aphid were investigated as two model systems to demonstrate the capability of high-resolution MSI based on MALDI. Localized molecular information on various plant- or pest-derived chemicals provided valuable insight for the molecular processes occurring during the plant–pest interactions. Basically, salicylic acid and isoflavone based resistance was visualized in the soybean–aphid system and antibiotic diterpenoids in rice–bacterium interactions.

Report Numbers

E 1.99:is-j--8639
is-j--8639

Subject(s)

Basic Biological Sciences

Note

Published through SciTech Connect.
04/27/2015.
"is-j--8639"
Analytical Chemistry 87 10 ISSN 0003-2700 AM
Klein, Adam; Yagnik, Gargey; Hohenstein, Jessica; Ji, Zhiyuan; Zi, Jiachen; Reichert, Malinda; MacIntosh, Gustavo; Yang, Bing; Peters, Reuben; Vela, Javier; Lee, Young.

Funding Information

2014-67013-21720
AC02-07CH11358

View MARC record | catkey: 23498438