Highly efficient Cas9-mediated transcriptional programming [electronic resource].
- Washington, D.C. : United States. Dept. of Energy, 2015. and Oak Ridge, Tenn. : Distributed by the Office of Scientific and Technical Information, U.S. Dept. of Energy
- Physical Description:
- pages 326-328 : digital, PDF file
- Additional Creators:
- Harvard University, United States. Department of Energy, National Institutes of Health (U.S.), National Cancer Institute (U.S.), National Science Foundation (U.S.), and United States. Department of Energy. Office of Scientific and Technical Information
- Restrictions on Access:
- Free-to-read Unrestricted online access
- The RNA-guided nuclease Cas9 can be reengineered as a programmable transcription factor. However, modest levels of gene activation have limited potential applications. Here we describe an improved transcriptional regulator through the rational design of a tripartite activator, VP64-p65-Rta (VPR), fused to nuclease-null Cas9. Here, we demonstrate its utility in activating endogenous coding and non-coding genes, targeting several genes simultaneously and stimulating neuronal differentiation of human induced pluripotent stem cells (iPSCs).
- Published through SciTech Connect., 03/02/2015., "nmeth.3312", Nature Methods 12 4 ISSN 1548-7091 AM, and Alejandro Chavez; Jonathan Scheiman; Suhani Vora; Benjamin W. Pruitt; Marcelle Tuttle; Eswar P. R. Iyer; Shuailiang Lin; Samira Kiani; Christopher D. Guzman; Daniel J. Wiegand; Dmitry Ter-Ovanesyan; Jonathan L. Braff; Noah Davidsohn; Benjamin E. Housden; Norbert Perrimon; Ron Weiss; John Aach; James J. Collins; George M. Church.
- Funding Information:
- FG02-02ER63445, 5T32CA009216-34, and P50 HG005550
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