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A technique for lyopreservation of <i>Clostridium ljungdahlii</i> in a biocomposite matrix for CO absorption [electronic resource].

Published
Washington, D.C. : United States. Dept. of Energy. Office of Energy Efficiency and Renewable Energy, 2017.
Oak Ridge, Tenn. : Distributed by the Office of Scientific and Technical Information, U.S. Dept. of Energy
Physical Description
Article numbers e0,180,806 : digital, PDF file
Additional Creators
United States. Department of Energy. Office of Energy Efficiency and Renewable Energy and United States. Department of Energy. Office of Scientific and Technical Information
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Summary
A system capable of biocatalytic conversion of distributed sources of single carbon gases such as carbon monoxide into hydrocarbons can be highly beneficial for developing commercially viable biotechnology applications in alternative energy. Several anaerobic bacterial strains can be used for such conversion. The anaerobic carbon monoxide-fixing bacteria Clostridium ljungdahlii OTA1 is a model CO assimilating microorganism that currently requires cryogenic temperature for storage of the viable strains. If these organisms can be stabilized and concentrated in thin films in advanced porous materials, it will enable development of high gas fraction, biocomposite absorbers with elevated carbon monoxide (CO) mass transfer rate, that require minimal power input and liquid, and demonstrate elevated substrate consumption rate compared to conventional suspended cell bioreactors. We report development of a technique for dry-stabilization of C. ljungdahlii OTA1 on a paper biocomposite. Bacterial samples coated onto paper were desiccated in the presence of trehalose using convective drying and stored at 4°C. Optimal dryness was ~1g H2O per gram of dry weight (gDW). CO uptake directly following biocomposite rehydration steadily increases over time indicating immediate cellular metabolic recovery. A high-resolution Raman microspectroscopic hyperspectral imaging technique was employed to spatially quantify the residual moisture content. We have demonstrated for the first time that convectively dried and stored C. ljungdahlii strains were stabilized in a desiccated state for over 38 days without a loss in CO absorbing reactivity. The Raman hyperspectral imaging technique described here is a non-invasive characterization tool to support development of dry-stabilization techniques for microorganisms on inexpensive porous support materials. In conclusion, the present study successfully extends and implements the principles of dry-stabilization for preservation of strictly anaerobic bacteria as an alternative to lyophilization or spray drying that could enable centralized biocomposite biocatalyst fabrication and decentralized bioprocessing of CO to liquid fuels or chemicals.
Report Numbers
E 1.99:1393201
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Note
Published through SciTech Connect.
07/05/2017.
PLoS ONE 12 7 ISSN 1932-6203 AM
Mark J. Schulte; Jason Solocinski; Mian Wang; Michelle Kovacs; Ryan Kilgore; Quinn Osgood; Lukas Underwood; Michael C. Flickinger; Nilay Chakraborty; Xiaoming He.
North Carolina State Univ., Raleigh, NC (United States)
Univ. of Michigan, Dearborn, MI (United States)
Funding Information
EE0006877
SusChEM 1510072
CHE 1609440
T32 GM008776-11
U038726
U046888
View MARC record | catkey: 23498558