Actions for The Yeast Polo Kinase Cdc5 Regulates the Shape of the Mitotic Nucleus [electronic resource].

Email RIS file
Bookmark
Report an Issue

The Yeast Polo Kinase Cdc5 Regulates the Shape of the Mitotic Nucleus [electronic resource].

Published
Washington, D.C. : United States. Dept. of Energy. Office of Science, 2014.
Oak Ridge, Tenn. : Distributed by the Office of Scientific and Technical Information, U.S. Dept. of Energy
Physical Description
pages 2,861-2,867 : digital, PDF file
Additional Creators
Lawrence Berkeley National Laboratory, United States. Department of Energy. Office of Science, and United States. Department of Energy. Office of Scientific and Technical Information
Access Online

Availability

I Want It
I Want It

Finding items...

Restrictions on Access
Free-to-read Unrestricted online access
Summary
Abnormal nuclear size and shape are hallmarks of aging and cancer. However, the mechanisms regulating nuclear morphology and nuclear envelope (NE) expansion are poorly understood. In metazoans, the NE disassembles prior to chromosome segregation and reassembles at the end of mitosis. In budding yeast, the NE remains intact. The nucleus elongates as chromosomes segregate and then divides at the end of mitosis to form two daughter nuclei without NE disassembly. The budding yeast nucleus also undergoes remodeling during a mitotic arrest; the NE continues to expand despite the pause in chromosome segregation, forming a nuclear extension, or "flare," that encompasses the nucleolus. The distinct nucleolar localization of the mitotic flare indicates that the NE is compartmentalized and that there is a mechanism by which NE expansion is confined to the region adjacent to the nucleolus. Here we show that mitotic flare formation is dependent on the yeast polo kinase Cdc5. This function of Cdc5 is independent of its known mitotic roles, including rDNA condensation. High-resolution imaging revealed that following Cdc5 inactivation, nuclei expand isometrically rather than forming a flare, indicating that Cdc5 is needed for NE compartmentalization. Lastly, even in an uninterrupted cell cycle, a small NE expansion occurs adjacent to the nucleolus prior to anaphase in a Cdc5-dependent manner. Our data provide the first evidence that polo kinase, a key regulator of mitosis, plays a role in regulating nuclear morphology and NE expansion.
Report Numbers
E 1.99:1257828
Subject(s)
Note
Published through SciTech Connect.
11/20/2014.
": S0960982214013384"
Current Biology 24 23 ISSN 0960-9822 AM
Alison D. Walters; Christopher K. May; Emma S. Dauster; Bertrand P. Cinquin; Elizabeth A. Smith; Xavier Robellet; Damien D’Amours; Carolyn A. Larabell; Orna Cohen-Fix.
Funding Information
AC02-05CH11231
MOP 82912
MOP 136788
P41GM103445
View MARC record | catkey: 23498686