Actions for Cell-free system for synthesizing membrane proteins cell free method for synthesizing membrane proteins [electronic resource].

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Cell-free system for synthesizing membrane proteins cell free method for synthesizing membrane proteins [electronic resource].

Published
Washington, D.C. : United States. Dept. of Energy, 2013.
Oak Ridge, Tenn. : Distributed by the Office of Scientific and Technical Information, U.S. Dept. of Energy
Additional Creators
Argonne National Laboratory, United States. Department of Energy, and United States. Department of Energy. Office of Scientific and Technical Information
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Summary
The invention provides an in vitro method for producing proteins, membrane proteins, membrane-associated proteins, and soluble proteins that interact with membrane-associated proteins for assembly into an oligomeric complex or that require association with a membrane for proper folding. The method comprises, supplying intracytoplasmic membranes from organisms; modifying protein composition of intracytoplasmic membranes from organism by modifying DNA to delete genes encoding functions of the organism not associated with the formation of the intracytoplasmic membranes; generating appropriate DNA or RNA templates that encode the target protein; and mixing the intracytoplasmic membranes with the template and a transcription/translation-competent cellular extract to cause simultaneous production of the membrane proteins and encapsulation of the membrane proteins within the intracytoplasmic membranes.
Report Numbers
E 1.99:8,455,231
8,455,231
Subject(s)
Note
Published through SciTech Connect.
06/04/2013.
"8,455,231"
"12/702,167"
Laible, Philip; Hanson, Deborah.
Funding Information
AC02-06CH11357
View MARC record | catkey: 23499343