Extracellular vesicles from <i>Paracoccidioides</i> pathogenic species transport polysaccharide and expose ligands for DC-SIGN receptors [electronic resource].

Published:: Washington, D.C. : United States. Dept. of Energy, 2015.
Oak Ridge, Tenn. : Distributed by the Office of Scientific and Technical Information, U.S. Dept. of Energy
Physical Description:: Article numbers 14,213 : digital, PDF file
Additional Creators:: United States. Department of Energy and United States. Department of Energy. Office of Scientific and Technical Information

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Summary:

Extracellular vesicles (EVs) mediate non-conventional transport of molecules across the fungal cell wall. We aimed at describing the carbohydrate composition and surface carbohydrate epitopes of EVs isolated from the pathogenic fungi Paracoccidioides brasiliensis and P. lutzii using standard procedures. Total EV carbohydrates were ethanol-precipitated from preparations depleted of lipids and proteins, then analyzed by chemical degradation, gas chromatography-mass spectrometry, nuclear magnetic resonance and size-exclusion chromatography. EV glycosyl residues of Glc, Man, and Gal comprised most probably two major components: a high molecular mass 4,6-α-glucan and a galactofuranosylmannan, possibly an oligomer, bearing a 2-α-Manp main chain linked to β-Galf (1,3) and α-Manp (1,6) end units. The results also suggested the presence of small amounts of a (1→6)- Manp polymer, (1→3)-glucan and (1→6)-glucan. Glycan microarrays allowed identification of EV surface lectin(s), while plant lectin microarray profiling revealed terminal Man and GlcNAc residues exposed at the EVs surface. Mammalian lectin microarray profiling showed that DC-SIGN receptors recognized surface carbohydrate in Paracoccidioides EVs. Our results suggest that oligosaccharides, cytoplasmic storage, and cell wall polysaccharides can be exported in fungal EVs, which also expose surface PAMPs and lectins. As a result, the role of these newly identified components in the interaction with the host remains to be unraveled.

Report Numbers:

E 1.99:1324971

Subject(s):

Basic Biological Sciences

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Note:

Published through SciTech Connect.
09/21/2015.
"srep14213"
Scientific Reports 5 ISSN 2045-2322 AM
Roberta Peres da Silva; Christian Heiss; Ian Black; Parastoo Azadi; Jared Q. Gerlach; Luiz R. Travassos; Lokesh Joshi; Michelle Kilcoyne; Rosana Puccia.
Univ. of Georgia, Athens, GA (United States). Analytical Services & Training Lab. Complex Carbohydrate Research Center

Funding Information:

FG02-93ER20097

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