Three dimensional time-gated tracking of non-blinking quantum dots in live cells [electronic resource].
- Washington, D.C. : United States. Dept. of Energy, 2015. and Oak Ridge, Tenn. : Distributed by the Office of Scientific and Technical Information, U.S. Dept. of Energy
- Physical Description:
- Article numbers 933,812 : digital, PDF file
- Additional Creators:
- Los Alamos National Laboratory, United States. Department of Energy, and United States. Department of Energy. Office of Scientific and Technical Information
- Restrictions on Access:
- Free-to-read Unrestricted online access
- Single particle tracking has provided a wealth of information about biophysical processes such as motor protein transport and diffusion in cell membranes. However, motion out of the plane of the microscope or blinking of the fluorescent probe used as a label generally limits observation times to several seconds. Here, we overcome these limitations by using novel non-blinking quantum dots as probes and employing a custom 3D tracking microscope to actively follow motion in three dimensions (3D) in live cells. As a result, signal-to-noise is improved in the cellular milieu through the use of pulsed excitation and time-gated detection.
- Published through SciTech Connect., 03/12/2015., "la-ur--15-20205", Proceedings of SPIE - The International Society for Optical Engineering 9338 ISSN 0277-786X AM, and Matthew S. DeVore; James H. Werner; Peter M. Goodwin; Aaron M. Keller; Jennifer A. Hollingsworth; Bridget S. Wilson; Cedric Cleyrat; Diane S. Lidke; Yagnaseni Ghosh; Michael H. Stewart; Dominik G. Stich; Mary E. Phipps.
- Funding Information:
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