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Overcoming substrate limitations for improved production of ethylene in <i>E. coli</i> [electronic resource].

Published:
Washington, D.C. : United States. Dept. of Energy. Office of Science, 2016.
Oak Ridge, Tenn. : Distributed by the Office of Scientific and Technical Information, U.S. Dept. of Energy
Physical Description:
10 pages : digital, PDF file
Additional Creators:
National Renewable Energy Laboratory (U.S.), United States. Department of Energy. Office of Science, and United States. Department of Energy. Office of Scientific and Technical Information
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Summary:
Ethylene is an important industrial compound for the production of a wide variety of plastics and chemicals. At present, ethylene production involves steam cracking of a fossil-based feedstock, representing the highest CO2-emitting process in the chemical industry. Biological ethylene production can be achieved via expression of a single protein, the ethylene-forming enzyme (EFE), found in some bacteria and fungi; it has the potential to provide a sustainable alternative to steam cracking, provided that significant increases in productivity can be achieved. A key barrier is determining factors that influence the availability of substrates for the EFE reaction in potential microbial hosts. In the presence of O2, EFE catalyzes ethylene formation from the substrates α-ketoglutarate (AKG) and arginine. The concentrations of AKG, a key TCA cycle intermediate, and arginine are tightly controlled by an intricate regulatory system that coordinates carbon and nitrogen metabolism. Thus, reliably predicting which genetic changes will ultimately lead to increased AKG and arginine availability is challenging.
Report Numbers:
E 1.99:nrel/ja--2700-63804
nrel/ja--2700-63804
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Note:
Published through SciTech Connect.
01/04/2016.
"nrel/ja--2700-63804"
Biotechnology for Biofuels 9 1 ISSN 1754-6834 AM
Sean Lynch; Carrie Eckert; Jianping Yu; Ryan Gill; Pin -Ching Maness.
Funding Information:
AC36-08GO28308
View MARC record | catkey: 23499809