Modular microfluidics for point-of-care protein purifications [electronic resource].
- Washington, D.C. : United States. Dept. of Energy. Office of Basic Energy Sciences, 2015. and Oak Ridge, Tenn. : Distributed by the Office of Scientific and Technical Information, U.S. Dept. of Energy
- Physical Description:
- pages 1,799-1,811 : digital, PDF file
- Additional Creators:
- Oak Ridge National Laboratory, United States. Department of Energy. Office of Basic Energy Sciences, United States. Defense Advanced Research Projects Agency, and United States. Department of Energy. Office of Scientific and Technical Information
- Biochemical separations are the heart of diagnostic assays and purification methods for biologics. On-chip miniaturization and modularization of separation procedures will enable the development of customized, portable devices for personalized health-care diagnostics and point-of-use production of treatments. In this report, we describe the design and fabrication of miniature ion exchange, size exclusion and affinity chromatography modules for on-chip clean-up of recombinantly-produced proteins. Our results demonstrate that these common separations techniques can be implemented in microfluidic modules with performance comparable to conventional approaches. We introduce embedded 3-D microfluidic interconnects for integrating micro-scale separation modules that can be arranged and reconfigured to suit a variety of fluidic operations or biochemical processes. In conclusion, we demonstrate the utility of the modular approach with a platform for the enrichment of enhanced green fluorescent protein (eGFP) from Escherichia coli lysate through integrated affinity and size-exclusion chromatography modules.
- Published through SciTech Connect., 01/01/2015., "453040135", Lab on a chip (Print) 15 8 ISSN 1473-0197; LCAHAM AM, and L. J. Millet; J. D. Lucheon; R. F. Standaert; S. T. Retterer; M. J. Doktycz.
- Funding Information:
- AC05-00OR22725 and HR001134005
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