Serial femtosecond X-ray diffraction of enveloped virus microcrystals [electronic resource].
- Washington, D.C. : United States. Dept. of Energy. Office of Science, 2015. and Oak Ridge, Tenn. : Distributed by the Office of Scientific and Technical Information, U.S. Dept. of Energy
- Physical Description:
- Article numbers 041,720 : digital, PDF file
- Additional Creators:
- SLAC National Accelerator Laboratory, United States. Department of Energy. Office of Science, and United States. Department of Energy. Office of Scientific and Technical Information
- Restrictions on Access:
- Free-to-read Unrestricted online access
- Serial femtosecond crystallography (SFX) using X-ray free-electron lasers has produced high-resolution, room temperature, time-resolved protein structures. We report preliminary SFX of Sindbis virus, an enveloped icosahedral RNA virus with ~700 Å diameter. Microcrystals delivered in viscous agarose medium diffracted to ~40 Å resolution. Small-angle diffuse X-ray scattering overlaid Bragg peaks and analysis suggests this results from molecular transforms of individual particles. Viral proteins undergo structural changes during entry and infection, which could, in principle, be studied with SFX. This is a pertinent step toward determining room temperature structures from virus microcrystals that may enable time-resolved studies of enveloped viruses.
- Published through SciTech Connect., 08/20/2015., Structural Dynamics 2 4 ISSN 2329-7778; SDTYAE AM, and Lawrence, Robert; Conrad, Chelsie; Zatsepin, Nadia; Grant, Thomas; Liu, Haiguang; James, Daniel; Nelson, Garrett; Subramanian, Ganesh; Aquila, Andrew; Hunter, Mark; Liang, Mengning; Boutet, Sébastien; Coe, Jesse; Spence, John; Weierstall, Uwe; Liu, Wei; Fromme, Petra; Cherezov, Vadim; Hogue, Brenda.
- Funding Information:
View MARC record | catkey: 23500144