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Simultaneous cryo X-ray ptychographic and fluorescence microscopy of green algae [electronic resource].

Published
Washington, D.C. : United States. Dept. of Energy. Office of Science, 2015.
Oak Ridge, Tenn. : Distributed by the Office of Scientific and Technical Information, U.S. Dept. of Energy
Physical Description
pages 2,314-2,319 : digital, PDF file
Additional Creators
Argonne National Laboratory, United States. Department of Energy. Office of Science, and United States. Department of Energy. Office of Scientific and Technical Information
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Summary
Trace metals play important roles in normal and in disease-causing biological functions. X-ray fluorescence microscopy reveals trace elements with no dependence on binding affinities (unlike with visible light fluorophores) and with improved sensitivity relative to electron probes. However, X-ray fluorescence is not very sensitive for showing the light elements that comprise the majority of cellular material. Here we show that X-ray ptychography can be combined with fluorescence to image both cellular structure and trace element distribution in frozen-hydrated cells at cryogenic temperatures, with high structural and chemical fidelity. Ptychographic reconstruction algorithms deliver phase and absorption contrast images at a resolution beyond that of the illuminating lens or beam size. Using 5.2-keV X-rays, we have obtained sub–30-nm resolution structural images and ~90-nm–resolution fluorescence images of several elements in frozen-hydrated green algae. This combined approach offers a way to study the role of trace elements in their structural context.
Report Numbers
E 1.99:1215636
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Note
Published through SciTech Connect.
02/24/2015.
Proceedings of the National Academy of Sciences of the United States of America 112 8 ISSN 0027-8424 AM
Deng, Junjing; Vine, David; Chen, Si; Nashed, Youssef; Jin, Qiaoling; Phillips, Nicholas; Peterka, Tom; Ross, Rob; Vogt, Stefan; Jacobsen, Chris.
Funding Information
AC02-06CH11357
1R01GM104530
1S10RR029272-01
View MARC record | catkey: 23500475