Global Analysis of <i>Salmonella</i> Alternative Sigma Factor E on Protein Translation [electronic resource].
- Washington, D.C. : United States. Dept. of Energy, 2015.
Oak Ridge, Tenn. : Distributed by the Office of Scientific and Technical Information, U.S. Dept. of Energy
- Physical Description:
- pages 1,716-1,726 : digital, PDF file
- Additional Creators:
- Pacific Northwest National Laboratory (U.S.)
United States. Department of Energy
National Institutes of Health (U.S.)
United States. Department of Energy. Office of Scientific and Technical Information
- The alternative sigma factor E (σ<sup>E</sup>) is critical for response to extracytoplasmic stress in Salmonella. Extensive studies have been conducted on σ<sup>E</sup>-regulated gene expression, particularly at the transcriptional level. Increasing evidence suggests however that σ<sup>E</sup> may indirectly participate in post-transcriptional regulation. Here in this study, we conducted sample-matched global proteomic and transcriptomic analyses to determine the level of regulation mediated by σ<sup>E</sup> in Salmonella. We analysed samples from wild type and isogenic rpoE mutant Salmonella cultivated in three different conditions; nutrient-rich and conditions that mimic early and late intracellular infection. We found that 30% of the observed proteome was regulated by σ<sup>E</sup> combining all three conditions. In different growth conditions, σ<sup>E</sup> affected the expression of a broad spectrum of Salmonella proteins required for miscellaneous functions. Those involved in transport and binding, protein synthesis, and stress response were particularly highlighted. By comparing transcriptomic and proteomic data, we identified genes post-transcriptionally regulated by σ<sup>E</sup> and found that post-transcriptional regulation was responsible for a majority of changes observed in the σ<sup>E</sup>-regulated proteome. Further, comparison of transcriptomic and proteomic data from hfq mutant of Salmonella demonstrated that σ<sup>E</sup>–mediated post-transcriptional regulation was partially dependent on the RNA-binding protein Hfq.
- Published through SciTech Connect.
Journal of Proteome Research 14 4 ISSN 1535-3893 AM
Jie Li; Ernesto S. Nakayasu; Christopher C. Overall; Rudd C. Johnson; Afshan S. Kidwai; Jason E. McDermott; Charles Ansong; Fred Heffron; Eric D. Cambronne; Joshua N. Adkins.
- Funding Information:
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