<i>In cellulo</i> serial crystallography of alcohol oxidase crystals inside yeast cells [electronic resource].
- Washington, D.C. : United States. Dept. of Energy. Office of Science, 2016. and Oak Ridge, Tenn. : Distributed by the Office of Scientific and Technical Information, U.S. Dept. of Energy
- Physical Description:
- pages 88-95 : digital, PDF file
- Additional Creators:
- SLAC National Accelerator Laboratory, United States. Department of Energy. Office of Science, and United States. Department of Energy. Office of Scientific and Technical Information
- Restrictions on Access:
- Free-to-read Unrestricted online access
- <p>The possibility of using femtosecond pulses from an X-ray free-electron laser to collect diffraction data from protein crystals formed in their native cellular organelle has been explored. X-ray diffraction of submicrometre-sized alcohol oxidase crystals formed in peroxisomes within cells of genetically modified variants of the methylotrophic yeast <i>Hansenula polymorpha</i> is reported and characterized. Furthermore, the observations are supported by synchrotron radiation-based powder diffraction data and electron microscopy. Based on these findings, the concept of <i>in cellulo</i> serial crystallography on protein targets imported into yeast peroxisomes without the need for protein purification as a requirement for subsequent crystallization is outlined.</p>
- Published through SciTech Connect., 03/01/2016., ": S2052252515022927", IUCrJ 3 2 ISSN 2052-2525; IUCRAJ AM, and Arjen J. Jakobi; Daniel M. Passon; Kevin Knoops; Francesco Stellato; Mengning Liang; Thomas A. White; Thomas Seine; Marc Messerschmidt; Henry N. Chapman; Matthias Wilmanns.
- Funding Information:
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