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Mapping mechanical force propagation through biomolecular complexes [electronic resource].

Published
Washington, D.C. : United States. Dept. of Energy. Office of Science, 2015.
Oak Ridge, Tenn. : Distributed by the Office of Scientific and Technical Information, U.S. Dept. of Energy
Physical Description
pages 7,370-7,376 : digital, PDF file
Additional Creators
University of Illinois at Urbana-Champaign, United States. Department of Energy. Office of Science, and United States. Department of Energy. Office of Scientific and Technical Information
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Summary
In this paper, we employ single-molecule force spectroscopy with an atomic force microscope (AFM) and steered molecular dynamics (SMD) simulations to reveal force propagation pathways through a mechanically ultrastable multidomain cellulosome protein complex. We demonstrate a new combination of network-based correlation analysis supported by AFM directional pulling experiments, which allowed us to visualize stiff paths through the protein complex along which force is transmitted. Finally, the results implicate specific force-propagation routes nonparallel to the pulling axis that are advantageous for achieving high dissociation forces.
Report Numbers
E 1.99:1345019
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Note
Published through SciTech Connect.
08/11/2015.
Nano Letters 15 11 ISSN 1530-6984 AM
Constantin Schoeler; Rafael C. Bernardi; Klara H. Malinowska; Ellis Durner; Wolfgang Ott; Edward A. Bayer; Klaus Schulten; Michael A. Nash; Hermann E. Gaub.
Funding Information
AC02-05CH11231
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