Amplification of trace amounts of nucleic acids [electronic resource].

Published:: Washington, D.C. : United States. Dept. of Energy, 2008.
Oak Ridge, Tenn. : Distributed by the Office of Scientific and Technical Information, U.S. Dept. of Energy
Additional Creators:: Harvard University, United States. Department of Energy, and United States. Department of Energy. Office of Scientific and Technical Information

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Summary:

Methods of reducing background during amplification of small amounts of nucleic acids employ careful analysis of sources of low level contamination. Ultraviolet light can be used to reduce nucleic acid contaminants in reagents and equipment. "Primer-dimer" background can be reduced by judicious design of primers. We have shown clean signal-to-noise with as little as starting material as one single human cell (.about.6 picogram), E. coli cell (.about.5 femtogram) or Prochlorococcus cell (.about.3 femtogram).

Report Numbers:

E 1.99:7,387,876
7,387,876

Subject(s):

Basic Biological Sciences

Note:

Published through SciTech Connect.
06/17/2008.
"7,387,876"
"US patent application 11/066,559"
Church, George M. (Brookline, MA); Zhang, Kun (Brighton, MA).

Funding Information:

FG02-02ER63445

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