Identification of Coq11, a New Coenzyme Q Biosynthetic Protein in the CoQ-Synthome in <i>Saccharomyces cerevisiae</i> [electronic resource].
- Washington, D.C. : United States. Dept. of Energy. Office of Science, 2015. and Oak Ridge, Tenn. : Distributed by the Office of Scientific and Technical Information, U.S. Dept. of Energy
- Physical Description:
- pages 7,517-7,534 : digital, PDF file
- Additional Creators:
- United States. Department of Energy. Office of Science, National Institutes of Health (U.S.), National Science Foundation (U.S.), and United States. Department of Energy. Office of Scientific and Technical Information
- Restrictions on Access:
- Free-to-read Unrestricted online access
- Coenzyme Q (Q or ubiquinone) is a redox active lipid composed of a fully substituted benzoquinone ring and a polyisoprenoid tail and is required for mitochondrial electron transport. In the yeast Saccharomyces cerevisiae, Q is synthesized by the products of 11 known genes, COQ1–COQ9, YAH1, and ARH1. The function of some of the Coq proteins remains unknown, and several steps in the Q biosynthetic pathway are not fully characterized. Several of the Coq proteins are associated in a macromolecular complex on the matrix face of the inner mitochondrial membrane, and this complex is required for efficient Q synthesis. In this paper, we further characterize this complex via immunoblotting and proteomic analysis of tandem affinity-purified tagged Coq proteins. We show that Coq8, a putative kinase required for the stability of the Q biosynthetic complex, is associated with a Coq6-containing complex. Additionally Q<sub>6</sub> and late stage Q biosynthetic intermediates were also found to co-purify with the complex. A mitochondrial protein of unknown function, encoded by the YLR290C open reading frame, is also identified as a constituent of the complex and is shown to be required for efficient de novo Q biosynthesis. Finally, given its effect on Q synthesis and its association with the biosynthetic complex, we propose that the open reading frame YLR290C be designated COQ11.
- Published through SciTech Connect., 01/28/2015., Journal of Biological Chemistry 290 12 ISSN 0021-9258 AM, Christopher M. Allan; Agape M. Awad; Jarrett S. Johnson; Dyna I. Shirasaki; Charles Wang; Crysten E. Blaby-Haas; Sabeeha S. Merchant; Joseph A. Loo; Catherine F. Clarke., and Univ. of California, Los Angeles, CA (United States)
- Funding Information:
- FC02-02ER63421, S10RR024605, R01GM103479, MCB-1330803, and GM100753
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