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Modulation of medium-chain fatty acid synthesis in <i>Synechococcus</i> sp. PCC 7002 by replacing FabH with a <i>Chaetoceros</i> Ketoacyl-ACP synthase [electronic resource].

Published:
Washington, D.C. : United States. Dept. of Energy. Office of Basic Energy Sciences, 2016.
Oak Ridge, Tenn. : Distributed by the Office of Scientific and Technical Information, U.S. Dept. of Energy
Physical Description:
13 pages : digital, PDF file
Additional Creators:
Colorado School of Mines, United States. Department of Energy. Office of Basic Energy Sciences, and United States. Department of Energy. Office of Scientific and Technical Information
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Summary:
The isolation or engineering of algal cells synthesizing high levels of medium-chain fatty acids (MCFAs) is attractive to mitigate the high clouding point of longer chain fatty acids in algal based biodiesel. To develop a more informed understanding of MCFA synthesis in photosynthetic microorganisms, we isolated several algae from Great Salt Lake and screened this collection for MCFA accumulation to identify strains naturally accumulating high levels of MCFA. A diatom, Chaetoceros sp. GSL56, accumulated particularly high levels of C14 (up to 40%), with the majority of C14 fatty acids allocated in triacylglycerols. Using whole cell transcriptome sequencing and de novo assembly, putative genes encoding fatty acid synthesis enzymes were identified. Enzymes from this Chaetoceros sp. were expressed in the cyanobacterium Synechococcus sp. PCC 7002 to validate gene function and to determine whether eukaryotic enzymes putatively lacking bacterial evolutionary control mechanisms could be used to improve MCFA production in this promising production strain. Replacement of the Synechococcus 7002 native FabH with a Chaetoceros ketoacyl-ACP synthase Ill increased MCFA synthesis up to fivefold. In conclusion, the level of increase is dependent on promoter strength and culturing conditions.
Report Numbers:
E 1.99:1281962
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Note:
Published through SciTech Connect.
05/26/2016.
Frontiers in Plant Science 7 ISSN 1664-462X AM
Huiya Gu; Robert E. Jinkerson; Fiona K. Davies; Lyle A. Sisson; Philip E. Schneider; Matthew C. Posewitz.
Funding Information:
SC0008547
View MARC record | catkey: 23501064