Molecular biological enhancement of coal biodesulfurization [electronic resource] : Second quarter report, January--April 1989

Published:: Washington, D.C. : United States. Dept. of Energy, 1989.
Oak Ridge, Tenn. : Distributed by the Office of Scientific and Technical Information, U.S. Dept. of Energy
Physical Description:: Pages: 31 : digital, PDF file
Additional Creators:: Institute of Gas Technology
United States. Department of Energy
United States. Department of Energy. Office of Scientific and Technical Information
Access Online:: www.osti.gov

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Summary:

The work planned for this quarter included the isolation of pure bacterial cultures capable of desulfurizing organic substrates and the genetic study of those cultures through the isolation and analysis of mutations. All aspects of the project are proceeding well and are either on or ahead of schedule. Two pure cultures of bacteria, that are each capable of utilizing dibenzothiophene (DBT) as their sole source of sulfur, were isolated from the mixed culture IGTS7. These cultures have been identified as Rhodococcus rhodochrous and Bacillus sphaericus species and have been designated IGTS8 and IGTS9, respectively. The examination of all of these cultures confirmed the identities of IGTS8 and IGTS9 as Rhodococcus rhodochrous and Bacillus sphaericus, respectively, and revealed that IGTS8 and IGTS9 are apparently unique in their ability to utilize organically-bound sulfur. The metabolites of DBT produced by IGTS8 were analyzed by gas chromatography/mass spectroscopy. Genetic studies of IGTS8 have begun. Mutants resistant to high levels of three different antibiotics, and combinations of antibiotics have been obtained. Resistance to antibiotics is a selectable genetic trait that will help to unequivocally identify this particular strain of bacteria and will be used in future genetic experiments. Chemical mutagenesis and ultra violet light mutagenesis procedures are being optimized. 2 figs., 8 tabs.

Subject(s):

Note:

Published through SciTech Connect.
04/01/1989.
"doe/pc/88891-t1"
"DE89011388"
Bielaga, B.; Kilbane, J. J.

Funding Information:

AC22-88PC88891

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