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Cathodoluminescence--detailed understanding may yield in situ distributions of individual molecular species at 100 A resolution or below [electronic resource].

Published:
Upton, N.Y. : Brookhaven National Laboratory, 1977.
Oak Ridge, Tenn. : Distributed by the Office of Scientific and Technical Information, U.S. Dept. of Energy
Physical Description:
Pages: 7 : digital, PDF file
Additional Creators:
Brookhaven National Laboratory and United States. Department of Energy. Office of Scientific and Technical Information
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Summary:
Of the several fluorescences of biological molecules excited under the scanning electron microscope and studied by us previously only the fluorescence of polymer nucleic acid appears long-lived enough to be considered a genuinely high-resolution probe. Since then, however, additional long-lived fluorophors have been uncovered or constructed, one a label for fluorescent antibody of potentially wide applicability. We discuss the knowns and unknowns of electron-induced fluorescence and detail our search for significant fluorophors of adequate lifetime under the beam. We review our instrumentation and compare it to that recently described by Steyn, Giles and Holt. Our mirror may provide appreciably better light collection. Our liquid-helium cold stage is described briefly and the importance of ultra-high vacuum emphasized. Preliminary results on vibrational structure in nucleic acid fluorescence at low temperature are presented. The potential utility of vibrational structure for separating single- and double-stranded nucleic acid and for localizing nascent DNA is noted. Ionic fluorescence, from rare-earth complexes and as observed in a preparation of poly-L-lysine, is discussed briefly. We present evidence for few-hundred A resolution in a fluorescence micrograph of a chromosome and outline our program for attempting to reach the 20 A-or-so ultimate resolution set by the range of fluorophor excitation.
Report Numbers:
E 1.99:bnl-22260
E 1.99: conf-770333-1
conf-770333-1
bnl-22260
Subject(s):
Note:
Published through SciTech Connect.
01/01/1977.
"bnl-22260"
" conf-770333-1"
IITRI scanning electron microscopy symposium, Chicago, IL, USA, 29 Mar 1977.
Hough, P.V.C.
Funding Information:
EY-76-C-02-0016
View MARC record | catkey: 23501517