Actions for Degradation of phenolic compounds by the lignocellulose deconstructing thermoacidophilic bacterium <i>Alicyclobacillus Acidocaldarius<

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Degradation of phenolic compounds by the lignocellulose deconstructing thermoacidophilic bacterium <i>Alicyclobacillus Acidocaldarius</i> [electronic resource].

Published
Washington, D.C. : United States. Dept. of Energy, 2015.
Oak Ridge, Tenn. : Distributed by the Office of Scientific and Technical Information, U.S. Dept. of Energy
Physical Description
pages 13-23 : digital, PDF file
Additional Creators
Idaho National Laboratory, United States. Department of Energy, and United States. Department of Energy. Office of Scientific and Technical Information
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Summary
Alicyclobacillus acidocaldarius, a thermoacidophilic bacterium, has a repertoire of thermo- and acid-stable enzymes that deconstruct lignocellulosic compounds. The work presented here describes the ability of A. acidocaldarius to reduce the concentration of the phenolic compounds: phenol, ferulic acid, ρ-coumaric acid and sinapinic acid during growth conditions. The extent and rate of the removal of these compounds were significantly increased by the presence of micro-molar copper concentrations, suggesting activity by copper oxidases that have been identified in the genome of A. acidocaldarius. Substrate removal kinetics was first order for phenol, ferulic acid, ρ-coumaric acid and sinapinic acid in the presence of 50 μM copper sulfate. In addition, laccase enzyme assays of cellular protein fractions suggested significant activity on a lignin analog between the temperatures of 45 and 90 °C. As a result, this work shows the potential for A. acidocaldarius to degrade phenolic compounds, demonstrating potential relevance to biofuel production and other industrial processes.
Report Numbers
E 1.99:inl/jou--13-30180
inl/jou--13-30180
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Note
Published through SciTech Connect.
11/05/2015.
"inl/jou--13-30180"
": 1700"
Journal of Industrial Microbiology and Biotechnology 43 1 ISSN 1367-5435 AM
John E. Aston; William A. Apel; Brady D. Lee; David N. Thompson; Jeffrey A. Lacey; Deborah T. Newby; David. W. Reed; Vicki S. Thompson.
Funding Information
AC07-05ID14517
View MARC record | catkey: 23502175