Fermentation of dilute acid pretreated <i>Populus</i> by <i>Clostridium thermocellum</i>, <i>Caldicellulosiruptor bescii</i>, and <i>Caldicellulosiruptor obsidiansis</i> [electronic resource].
- Washington, D.C. : United States. Dept. of Energy. Office of Science, 2015. and Oak Ridge, Tenn. : Distributed by the Office of Scientific and Technical Information, U.S. Dept. of Energy
- Physical Description:
- pages 1,014-1,021 : digital, PDF file
- Additional Creators:
- Oak Ridge National Laboratory, United States. Department of Energy. Office of Science, and United States. Department of Energy. Office of Scientific and Technical Information
- Restrictions on Access:
- Free-to-read Unrestricted online access
- Consolidated bioprocessing (CBP), which merges enzyme production, biomass hydrolysis, and fermentation into a single step, has the potential to become an efficient and economic strategy for the bioconversion of lignocellulosic feedstocks to transportation fuels or chemicals. In this study, we evaluated <i>Clostridium thermocellum</i>, <i>Caldicellulosiruptor bescii</i>, and <i>Caldicellulosiruptor obsidiansis</i>, three , thermophilic,cellulolytic, mixed-acid fermenting candidate CBP microorganisms, for their fermentation capabilities using dilute acid pretreated <i>Populus</i> as a model biomass feedstock. Under pH controlled, anaerobic fermentation conditions, each candidate successfully digested a minimum of 75% of the cellulose from dilute acid pretreated <i>Populus</i>, as indicated by an increase in planktonic cells and end-product metabolites and a concurrent decrease in glucan content. <i>C. thermocellum</i>, which employs a cellulosomal approach to biomass degradation, required 120 hours to achieve 75% cellulose utilization. In contrast, the non-cellulosomal, secreted hydrolytic enzyme system of the <i>Caldicellulosiruptor</i> sp. required 300 hours to achieve similar results. End-point fermentation conversions for <i>C. thermocellum</i>, <i>C. bescii</i>, and <i>C. obsidiansis</i> were determined to be 0.29, 0.34, and 0.38 grams of total metabolites per gram of loaded glucan, respectively. This data provide a starting point for future strain engineering efforts that can serve to improve the biomass fermentation capabilities of these three promising candidate CBP platforms.
- Published through SciTech Connect., 07/25/2015., "KP1601050", "ERKP695", BioEnergy Research 8 3 ISSN 1939-1234 AM, San Jose, California United States, 8-13 June 2014., and Yee, Kelsey; Rodriguez, Jr., Miguel; Hamilton, Choo Yieng [ORNL]; Hamilton-Brehm, Scott; Thompson, Olivia; Elkins, James; Davison, Brian; Mielenz, Jonathan.
- Funding Information:
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