Actions for Interlaboratory comparison of mutagenesis testing of coal fly ash derived from different coal conversion technologies [electronic resource].

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Interlaboratory comparison of mutagenesis testing of coal fly ash derived from different coal conversion technologies [electronic resource].

Published
Washington, D.C. : United States. Dept. of Energy, 1979.
Oak Ridge, Tenn. : Distributed by the Office of Scientific and Technical Information, U.S. Dept. of Energy
Physical Description
Pages: 38 : digital, PDF file
Additional Creators
Argonne National Laboratory, United States. Department of Energy, and United States. Department of Energy. Office of Scientific and Technical Information
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Summary
This experiment showed that mutagenicity of fly ash derived from different coal conversion technologies, as determined by the Ames plate incorporation test, was similar in all three laboratories. The differences in mutagenic activity of each fly ash between laboratories with different solvent extraction methods were no greater than one order of magnitude. In addition, there were much smaller, but still significant differences in mutagenic activity between laboratories when the same solvent extract of a particular fly ash was tested in each laboratory. There were also significant differences in mutagenicity of the positive control mutagen (maximum of fivefold) between laboratories. Because of this difference in Ames test sensitivity between laboratories, the influence of the solvent extraction methods on differences in mutagenicity was not clear. However, the data suggested that either there were significant differences in the degree of sensitivity of Ames tests for different complex mixtures within each laboratory, or else there were differences in mutagen extraction efficiency between different solvent extraction methods. Both Ames test sensitivity and solvent extraction may be important. Further work would be necessary to separate the contribution of these two factors. An important aspect of further work would be to separate the contribution of the innate sensitivity of substrains of Ames tester strains in each laboratory from the possible effects of differences in Ames testing methodology. This could be done by testing the same extracts of fly ash and positive control mutagens with substrains of tester strains exchanged between laboratories. This work also implies that caution should be exercised in assuming that the same solvent would have the same efficiency for extraction of mutagens from different fly ashes even within the same laboratory.
Report Numbers
E 1.99:conf-791002-4
conf-791002-4
Subject(s)
Note
Published through SciTech Connect.
01/01/1979.
"conf-791002-4"
19. annual Hanford life sciences symposium, Richland, WA, USA, 22 Oct 1979.
Chrisp, C.; Hobbs, C.; Clark, R.; Kubitschek, H.E.
Funding Information
W-31-109-ENG-38
View MARC record | catkey: 23505695