Introduction to macromolecular crystallography / Alexander McPherson
- McPherson, Alexander, 1944-
- Hoboken, N.J. : Wiley-Liss, 
- Copyright Date:
- Physical Description:
- x, 237 pages : illustrations ; 26 cm
- An overview of macromolecular crystallography. What do we mean by the structure of something? -- An analogy -- A lens -- How x-ray diffraction works -- The phase problem -- The electron density -- The nature of crystals: symmetry and the unit cell. The asymmetric unit -- The space group -- The unit cell -- The lattice translations -- Symmetry and equivalent positions -- Why so few kinds of unit cells -- Primitive and centered lattices -- Planes, Miller indices, and convolutions -- The reciprocal lattice -- Crystals as waves of electrons in three-dimensional space -- Waves and their properties. Waves as vectors and complex numbers -- Addition of waves -- Vector arithmetic -- Some useful wave relationships -- The Fourier synthesis, planes and the electron density -- Diffraction from points, planes, molecules and crystals. The diffraction pattern from arbitrary array of points in space -- Diffraction from equally spaced points along a line -- Diffraction from a plane, families of planes and lattices of points -- Continuous and discontinuous transforms -- The structure factor equation for a crystal -- Temperature factors -- Center of symmetry -- Friedel's law and anomalous scattering -- Anomalous dispersion effects -- The electron density equation -- Examination of diffraction patterns -- Ewald's sphere and Bragg's law -- Crystal symmetry and the symmetry of the diffraction pattern -- Symmetry and systematic absences -- Analysis of diffraction patterns -- More thoughts on space groups -- Solving the phase problem. Patterson methods -- The heavy atom method -- The R factor and crystallographic refinement -- Isomorphous replacement -- Formulation of isomorphous replacement in protein crystallography -- Isomorphous replacement in practice -- Molecular replacement -- Phase extension using noncrystallographic symmetry -- Anomalous scattering approaches -- Direct methods -- Interpreting Patterson maps. Interpreting the Patterson map -- Harker vectors and peaks -- Using the Patterson map for isomorphous replacement -- Electron density maps and difference Fourier maps -- Interpretation -- Criteria for judging a structure determination -- Biochemical experiments using x-ray crystallography -- The difference Fourier method.
- 0471251224 (acid-free paper)
- Bibliography Note:
- Includes bibliographical references (pages 227-229) and index.
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