Actions for Final Report [electronic resource] : Molecular mechanisms and kinetics of microbial anaerobic nitrate-dependent U(IV) and Fe(II) oxidation
Final Report [electronic resource] : Molecular mechanisms and kinetics of microbial anaerobic nitrate-dependent U(IV) and Fe(II) oxidation
- Published
- Washington, D.C. : United States. Dept. of Energy. Office of Science, 2015.
Oak Ridge, Tenn. : Distributed by the Office of Scientific and Technical Information, U.S. Dept. of Energy - Physical Description
- 3 pages : digital, PDF file
- Additional Creators
- United States. Department of Energy. Office of Science and United States. Department of Energy. Office of Scientific and Technical Information
Access Online
- Restrictions on Access
- Free-to-read Unrestricted online access
- Summary
- In this project, we combined molecular genetic, spectroscopic, and microscopic techniques with kinetic and reactive transport studies to describe and quantify biotic and abiotic mechanisms underlying anaerobic, nitrate-dependent U(IV) and Fe(II) oxidation, which influences the long-term efficacy of in situ reductive immobilization of uranium at DOE sites. In these studies, Thiobacillus denitrificans, an autotrophic bacterium that catalyzes anaerobic U(IV) and Fe(II) oxidation, was used to examine coupled oxidation-reduction processes under either biotic (enzymatic) or abiotic conditions in batch and column experiments with biogenically produced UIVO2(s). Synthesis and quantitative analysis of coupled chemical and transport processes were done with the reactive transport modeling code Crunchflow. Research focused on identifying the primary redox proteins that catalyze metal oxidation, environmental factors that influence protein expression, and molecular-scale geochemical factors that control the rates of biotic and abiotic oxidation.
- Report Numbers
- E 1.99:doe-ucm--biou
doe-ucm--biou - Subject(s)
- Other Subject(s)
- Note
- Published through SciTech Connect.
02/27/2015.
"doe-ucm--biou"
Peggy A. O'Day; Maria P. Asta; Masakazu Kanematsu; Harry Beller; Peng Zhou; Carl Steefel.
Univ. of California, Merced, CA (United States) - Type of Report and Period Covered Note
- Final;
- Funding Information
- SC0005479
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