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Simultaneous knockdown of six non-family genes using a single synthetic RNAi fragment in Arabidopsis thaliana [electronic resource].

Published
Washington, D.C. : United States. Dept. of Energy. Office of Science, 2016.
Oak Ridge, Tenn. : Distributed by the Office of Scientific and Technical Information, U.S. Dept. of Energy
Physical Description
Article numbers 16 : digital, PDF file
Additional Creators
Oak Ridge National Laboratory, United States. Department of Energy. Office of Science, and United States. Department of Energy. Office of Scientific and Technical Information
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Summary
Genetic engineering of plants that results in successful establishment of new biochemical or regulatory pathways requires stable introduction of one or more genes into the plant genome. It might also be necessary to down-regulate or turn off expression of endogenous genes in order to reduce activity of competing pathways. An established way to knockdown gene expression in plants is expressing a hairpin-(hp)-RNAi construct, eventually leading to degradation of a specifically targeted mRNA. Ultimately, knockdown of multiple genes that do not share homologous sequences is still challenging and involves either sophisticated cloning strategies to create vectors with different serial expression constructs or multiple transformation events that is often restricted by a lack of available transformation markers.
Report Numbers
E 1.99:1311232
Subject(s)
Note
Published through SciTech Connect.
02/17/2016.
"KP1601010"
"ERKP831"
Plant Methods 12 1 ISSN 1746-4811 AM
Olaf Czarnecki; Anthony C. Bryan; Sara S. Jawdy; Xiaohan Yang; Zong-Ming Cheng; Jin-Gui Chen; Gerald A. Tuskan.
Funding Information
AC05-00OR22725
SC0008834
View MARC record | catkey: 23782447