Actions for Improving the Sensitivity of Mass Spectrometry by Using a New Sheath Flow Electrospray Emitter Array at Subambient Pressures [electronic resource].

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Improving the Sensitivity of Mass Spectrometry by Using a New Sheath Flow Electrospray Emitter Array at Subambient Pressures [electronic resource].

Published
Washington, D.C. : United States. Dept. of Energy. Office of Science, 2014.
Oak Ridge, Tenn. : Distributed by the Office of Scientific and Technical Information, U.S. Dept. of Energy
Physical Description
pages 2,028-2,037 : digital, PDF file
Additional Creators
Pacific Northwest National Laboratory (U.S.), United States. Department of Energy. Office of Science, and United States. Department of Energy. Office of Scientific and Technical Information
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Summary
Arrays of chemically etched emitters with individualized sheath gas capillaries have been developed to enhance electrospray ionization (ESI) at subambient pressures. By including an emitter array in a subambient pressure ionization with nanoelectrospray (SPIN) source, ionization and transmission efficiency can be maximized allowing for increased sensitivity in mass spectrometric analyses. The SPIN source eliminates the major ion losses at conventional ESI-mass spectrometry (MS) interface by placing the emitter in the first vacuum region of the instrument. To facilitate stable electrospray currents in such conditions we have developed an improved emitter array with individualized sheath gas around each emitter. The utility of the new emitter arrays for generating stable multi-electrosprays at subambient pressures was probed by coupling the emitter array/SPIN source with a time of flight (TOF) mass spectrometer. The instrument sensitivity was compared between single emitter/SPIN-MS and multi-emitter/SPIN-MS configurations using an equimolar solution of 9 peptides. An increase in sensitivity correlative to the number of emitters in the array was observed.
Report Numbers
E 1.99:pnnl-sa--96045
pnnl-sa--96045
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Note
Published through SciTech Connect.
03/28/2014.
"pnnl-sa--96045"
"48135"
"47301"
"400412000"
Journal of the American Society for Mass Spectrometry 25 12 ISSN 1044-0305 AM
Jonathan T. Cox; Ioan Marginean; Ryan T. Kelly; Richard D. Smith; Keqi Tang.
Funding Information
AC05-76RL01830
1R33CA155252
RR18522
8 P41 GM103493-10
AC05-76RLO01830
View MARC record | catkey: 24047223