An interbacterial NAD(P)<sup>+</sup> glycohydrolase toxin requires elongation factor Tu for delivery to target cells [electronic resource].
- Published:
- Washington, D.C. : United States. Dept. of Energy. Office of Basic Energy Sciences, 2015.
Oak Ridge, Tenn. : Distributed by the Office of Scientific and Technical Information, U.S. Dept. of Energy - Physical Description:
- pages 607-619 : digital, PDF file
- Additional Creators:
- Brookhaven National Laboratory, United States. Department of Energy. Office of Basic Energy Sciences, and United States. Department of Energy. Office of Scientific and Technical Information
Access Online
- Restrictions on Access:
- Free-to-read Unrestricted online access
- Summary:
- Type VI secretion (T6S) influences the composition of microbial communities by catalyzing the delivery of toxins between adjacent bacterial cells. Here, we demonstrate that a T6S integral membrane toxin from Pseudomonas aeruginosa, Tse6, acts on target cells by degrading the universally essential dinucleotides NAD+ and NADP+. Structural analyses of Tse6 show that it resembles mono-ADP-ribosyltransferase proteins, such as diphtheria toxin, with the exception of a unique loop that both excludes proteinaceous ADP-ribose acceptors and contributes to hydrolysis. We find that entry of Tse6 into target cells requires its binding to an essential housekeeping protein, translation elongation factor Tu (EF-Tu). These proteins participate in a larger assembly that additionally directs toxin export and provides chaperone activity. Lastly, visualization of this complex by electron microscopy defines the architecture of a toxin-loaded T6S apparatus and provides mechanistic insight into intercellular membrane protein delivery between bacteria.
- Report Numbers:
- E 1.99:bnl--112066-2016-ja
bnl--112066-2016-ja - Subject(s):
- Note:
- Published through SciTech Connect.
10/08/2015.
"bnl--112066-2016-ja"
Cell 163 3 ISSN 0092-8674 AM
John C. Whitney; Dennis Quentin; Shin Sawai; Michele LeRoux; Brittany N. Harding; Hannah E. Ledvina; Bao Q. Tran; Howard Robinson; Young Ah Goo; David R. Goodlett; Stefan Raunser; Joseph D. Mougous. - Funding Information:
- SC00112704
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