Control of membrane permeability in air-stable droplet interface bilayers [electronic resource].
- Published:
- Washington, D.C. : United States. Dept. of Energy. Office of Science, 2015.
Oak Ridge, Tenn. : Distributed by the Office of Scientific and Technical Information, U.S. Dept. of Energy - Physical Description:
- 4,224-4,231 : digital, PDF file
- Additional Creators:
- Oak Ridge National Laboratory, United States. Department of Energy. Office of Science, and United States. Department of Energy. Office of Scientific and Technical Information
Access Online
- Restrictions on Access:
- Free-to-read Unrestricted online access
- Summary:
- Air-stable droplet interface bilayers (airDIBs) on oil-infused surfaces are versatile model membranes for synthetic biology applications, including biosensing of airborne species. However, air-DIBs are subject to evaporation, which can, over time, destabilize them and reduce their useful lifetime compared to traditional DIBs that are fully submerged in oil. Here, we show that lifetimes of air-DIBs can be extended by as much as an order of magnitude by maintaining them at a temperature just above the dew point. We find that raising the temperature from near the dew point (7 C at 38.5 % relative humidity) to room temperature results in loss of water molecules of hydration from the polar head groups of the lipid bilayer membrane due to evaporation in an irreversible process that increases the overall entropy of the system. This dehydration transition affects primarily the bilayer resistance, by increasing ion permeability through the increasingly disordered polar head group region of the bilayer. Temperature and/or relative humidity are conveniently tunable parameters for controlling the stability and composition of air-DIBs membranes, while still allowing for operation in ambient environments.
- Report Numbers:
- E 1.99:1185818
- Subject(s):
- Note:
- Published through SciTech Connect.
03/19/2015.
"KC0403040"
"ERKCZ01"
Langmuir 31 14 ISSN 0743-7463 AM
Prachya Mruetusatorn; Georgios Polizos; Panos G. Datskos; Graham Taylor; Stephen A. Sarles; Jonathan Boreyko; Douglas G. Hayes; Pat Collier. - Funding Information:
- AC05-00OR22725
View MARC record | catkey: 24057522