- Restrictions on Access:
- Open Access.
- The six-transmembrane (6-TM) superfamily of ion channels is a diverse group of tetramer-forming ion channels. Members of this superfamily are regulated by a many different physical and chemical stimuli, including (but not limited to): organic compounds, temperature, mechanical stress, and transmembrane potential. 6-TM channels are critical for regulating neuronal excitability, cardiac pacemaking, sensing of thermal and mechanical stimuli, and maintaining water balance in plants among many other functions. In this thesis, I will first present an overview of 6-TM containing channels. This will be followed by three different projects involving channels from three families within the 6 TM superfamily: Shaker, Transient Receptor Potential Vanilloid (TRPV), and Cyclic Nucleotide Binding Domain (CNBD) channels. The project involving Shaker family channels identifies the stoichiometry of an obligatory heteromeric subunit and determines that this stoichiometry is regulated by a mechanism involving the activation gate. In the TRPV project, C. elegans and D. melanogaster TRPV channels are found to be activated by the vitamin B3 metabolite nicotinamide. In the investigations of CNBD containing channels, it is determined that heme appears to be an evolutionarily conserved regulator of CNBD-containing channels in both plant and animal lineages. Finally, I will discuss where these works fit into our current understanding of ion channels and physiology and will speculate on the outcome of new questions posed by the results of these three projects.
- Dissertation Note:
- Ph.D. Pennsylvania State University 2018.
- Technical Details:
- The full text of the dissertation is available as an Adobe Acrobat .pdf file ; Adobe Acrobat Reader required to view the file.
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