Actions for Development and Validation of a Detection Method for a Broad Range of Human Papillomavirus Types

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Development and Validation of a Detection Method for a Broad Range of Human Papillomavirus Types / DN. Galbraith, T. Collins, J. Black, B. McManus, D. McMutrie, A. Lovatt

Conference Author
Tissue Engineered Medical Products (TEMPs) (2002 : Miami Beach, Florida)
Physical Description
1 online resource (8 pages) : illustrations, figures, tables
Additional Creators
McMutrie, D., Black, John, 1931-, Collins, T, Galbraith, DN., Lovatt, A., McManus, B., American Society for Testing and Materials, and ASTM International
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Summary
Human papillomaviruses (HPVs) are viruses that are almost unique in their tropism for human skin cells; they also have the potential to cause severe pathology. These viruses present a significant risk to contaminate tissue engineered skin replacement products that use human-derived skin cells as part of their manufacture. Eight pairs of primers and appropriate DNA oligonucleotide probes were designed from a relatively conserved region of the LI open reading frame of HPV. TaqMan¿̐ư PCR using these primer/probe sets was found to be capable of detecting HPV-DNA in a background of cellular material likely to be present in skin replacement products. The assay is more sensitive than any currently described in the scientific literature and can be completed in a single working day making it suitable for testing the short shelf life that many of these products have.
Dates of Publication and/or Sequential Designation
Volume 2004, Issue 1452 (January 2004)
Subject(s)
Other Subject(s)
ISBN
9780803154971 (e-ISBN)
9780803134713
0803134711
Digital File Characteristics
text file PDF
Bibliography Note
Includes bibliographical references 9.
Other Forms
Also available online via the World Wide Web. Tables of contents and abstracts freely available; full-text articles available by subscription.
Full text article also available for purchase.
Also available in PDF edition.
Reproduction Note
Electronic reproduction. W. Conshohocken, Pa. : ASTM International, 2004. Mode of access: World Wide Web. System requirements: Web browser. Access may be restricted to users at subscribing institutions.
Technical Details
Mode of access: World Wide Web.
Source of Acquisition
ASTM International PDF Purchase price USD25.
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