Performance of an Enzyme Electrode Designed for a Sulfide Monitoring Biosensor / Reeta Sooknah, Sankara Papavinasam, Michael Attard, R. Winston Revie, W. Douglas Gould, Orlando Dinardo

Conference Author:: Advances in Electrochemical Techniques for Corrosion Monitoring and Measurement (2007 : Norfolk, VA)
Physical Description:: 1 online resource (14 pages) : illustrations, figures, tables
Additional Creators:: Sooknah, Reeta, Attard, Michael, Dinardo, Orlando, Gould, W. Douglas, Papavinasam, Sankara, 1962-, Revie, R. Winston, American Society for Testing and Materials, and ASTM International

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Summary:

The construction and evaluation of an enzyme electrode designed for a sulfide monitoring biosensor, are reported in this work. The enzyme, which is immobilized in a carbon (graphite) paste with 1,1 ¿̐ư dimeth-ylferrocene, catalyzes the oxidation of sulfide to sulfur.Amperometric measurements were carried out at room temperature, and a fixed potential of 0.3 V with reference to a saturated calomel electrode, using Tris-HCI buffer (pH 7.5) as the electrolyte, and sodium sulfide solution as the substrate. Under deaerated conditions, the biosensor responded linearly to the tested sulfide concentration range of 0¿̐ư4mM, giving an average apparent Michaelis constant of 3mM, and maximum current responses between 0.7 and 3.6 uA. The sensitivity of the electrodes tested ranged from 0.2¿̐ư0.3 ?A mM¿̐ư1.

Dates of Publication and/or Sequential Designation:

Volume 2009, Issue 1506 (January 2009)

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ISBN:

9780803169975 (e-ISBN)
9780803155220

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text file PDF

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Includes bibliographical references 26.

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Electronic reproduction. W. Conshohocken, Pa. : ASTM International, 2009. Mode of access: World Wide Web. System requirements: Web browser. Access may be restricted to users at subscribing institutions.

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Mode of access: World Wide Web.

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