Actions for Biochemical Measurements of Microbial Mass and Activity from Environmental Samples

Email RIS file
Bookmark
Report an Issue

Biochemical Measurements of Microbial Mass and Activity from Environmental Samples / DC. White, RJ. Bobbie, JS. Herron, JD. King, SJ. Morrison

Conference Author
Native Aquatic Bacteria: Enumeration, Activity, and Ecology (1977 : Minneapolis, Minn.)
Physical Description
1 online resource (13 pages) : illustrations, figures, tables
Additional Creators
White, DC., Bobbie, RJ., Herron, JS., King, JD., Morrison, SJ., American Society for Testing and Materials, and ASTM International
Access Online

Availability

I Want It
I Want It

Finding items...

Restrictions on Access
Subscription required for access to full text.
License restrictions may limit access.
Summary
Accurate measurement of the mass and activities of environmental microbial assemblies requires methods that differ from those of classical microbiology. Using the leaf-litter detrital microflora recovered after incubation in a semitropical estuary, biochemical assays of multiple components can be made which give reasonably similar estimates of microbial mass, using normalization values from bacterial monocultures. The assays involve recovery of adenosine triphosphate, the unique bacterial cell wall mucopeptide component muramic acid, the uniquely prokaryotic endogenous storage material poly-?-hydroxybutyrate, a series of exoesterase activities, and the extractable lipids, primarily the phospholipids and the glycolipids. If the sampling conditions are such that incorporation of radiosotopes into the microflora is possible, these assays become extraordinarily sensitive. If radioisotope usage can be coupled with the purification of particular lipids, it is possible to gain information on the structure and dynamics of the microbial population.
Dates of Publication and/or Sequential Designation
Volume 1979, Issue 695 (January 1979)
Subject(s)
Other Subject(s)
ISBN
9780803147607 (e-ISBN)
9780803105263
0803105266
Digital File Characteristics
text file PDF
Bibliography Note
Includes bibliographical references 50.
Other Forms
Also available online via the World Wide Web. Tables of contents and abstracts freely available; full-text articles available by subscription.
Full text article also available for purchase.
Also available in PDF edition.
Reproduction Note
Electronic reproduction. W. Conshohocken, Pa. : ASTM International, 1979. Mode of access: World Wide Web. System requirements: Web browser. Access may be restricted to users at subscribing institutions.
Technical Details
Mode of access: World Wide Web.
Source of Acquisition
ASTM International PDF Purchase price USD25.
View MARC record | catkey: 28007733