Actions for Bacterial polysaccharides [electronic resource] : methods and protocols
Bacterial polysaccharides [electronic resource] : methods and protocols / edited by Inka Brockhausen
- Uniform Title
- Bacterial polysaccharides (Brockhausen)
- Published
- New York, NY : Humana Press, [2019]
- Physical Description
- xiii, 312 pages : illustrations ; 27 cm.
- Additional Creators
- Brockhausen, Inka, 1944-
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- Series
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- License restrictions may limit access.
- Contents
- Customizable cloning of whole polysaccharide gene clusters by yeast homologous recombination / Michael A. Liu and Peter R. Reeves -- RecET direct cloning of polysaccharide gene cluster from gram-negative bacteria / Zhongrui Ma and Peng George Wang - Purification of capsular polysaccharide complex from gram-negative bacteria / Carlos A. Alvarez and Brian A. Cobb -- Simple protocol to purify cell wall polysaccharide from gram-positive bacteria and assess its structural integrity / Irina Sadovskaya and Yann Guerardel -- Extraction and purification of wall-bound polymers of gram-positive bacteria / Alice Chateau, Olaf Schneewind, and Dominique Missiakas -- Purification and analysis of mycobacterial phosphatidylinositol mannosides, lipomannan, and lipoarabinomannan / Kathryn C. Rahlwes, Julia Puffal, and Yasu S. Morita -- Conjugation of synthetic oligosaccharides to proteins by squaric acid chemistry / Helene B. Pfister, Xiaowei Lu, Sameh E. Soliman, and Pavol Kovac -- Direct conjugation of bacterial polysaccharides to proteins by squaric acid chemistry / Peng Xu and Pavol Kovac -- Chemical modification of the N-acetyl moieties of hyaluronic acid from Streptococcus equi for studies in cytokine production / Siziwe Bebe and Tassos Anastassiades -- Assays for the enzymes catalyzing the o-acetylation of bacterial cell wall polysaccharides / Ashley S. Brott, David Sychantha, and Anthony J. Clarke -- Designing glycosyltransferase expression constructs for improved purification, protein yield, and crystallization / Taylor J. B. Forrester, Lana El Osta, Joseph S. Lam, and Matthew S. Kimber -- Utilization of fluorescently tagged synthetic acceptor molecules for in vitro characterization of a dual-domain glycosyltransferase enzyme, kpsc, from Escherichia coli / Liam Doyle, Olga G. Ovchinnikova, and Chris Whitfield -- Synthesis of Phenoxyundecyl diphosphate disaccharides for studies of the biosynthesis of O antigenic polysaccharides in enteric bacteria / Vladimir Torgov, Leonid Danilov, Natalia Utkina, Vladimir Veselovsky, Alexander Kocev, and Inka Brockhausen -- Characterization of arabinosyl transfer reactions in the biosynthesis of mycobacterial cell envelope (lipo)polysaccharides / Shiva Kumar Angala and Mary Jackson -- Enzymatic synthesis of repeating unit oligosaccharides of Escherichia coli O104 / Inka Brockhausen and Diana Czuchry -- Sulfhydryl labeling as a tool to investigate the topology of membrane proteins involved in lipopolysaccharide biosynthesis / Faviola Tavares-Carreon, Xiang Ruan, Amy Ford, and Miguel A. Valvano -- Assay methods for the glycosyltransferases involved in synthesis of bacterial polysaccharides / Tasnim Abukar, Nakita Buenbrazo, Bettina Janesch, Laura Kell, and Warren Wakarchuk -- Glycosyltransferase activity assay using colorimetric methods / M. Shafiqur Rahman and Wensheng Qin -- In vitro characterization of a multidomain glycosyltransferase using fluorescently tagged synthetic acceptors / Danielle M. Williams, Olga G. Ovchinnikova, and Chris Whitfield -- Glycosyltransferase-coupled assays for 4-epimerase WbpP from Pseudomonas aeruginosa / Sulav Sharma, Carole Creuzenet, Kenneth F. Jarrell, and Inka Brockhausen -- Assaying fucosidase activity / Zoe Anne Megson, Paul Messner, and Christina Schaffer -- In vitro synthesis of bioactive glycovariants of enterocin 96, an antimicrobial peptide from Enterococcus faecalis / Rupa Nagar and Alka Rao -- High-throughput screening for inhibitors of wall teichoic acid biosynthesis in Staphylococcus aureus / Omar M. El-Halfawy and Eric D. Brown.
- Summary
- This book provides a selection of recently developed methods and protocols in bacterial glycomics to aid in bettering our understanding of the structures and functions of bacterial polysaccharides, their attachments to proteins and lipids, their role in biofilm formation, as well as their biosynthesis. With the emerging bacterial resistance to commonly used antibiotics world-wide, these techniques to study the outer polysaccharides of bacteria, with their functions in bacterial adhesion, colonization, growth, establishment of biofilms, and control virulence and pathogenicity, are increasingly important. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Bacterial Polysaccharides: Methods and Protocols aims to support researchers contributing to future approaches that will fill our knowledge gaps and define anti-bacterial targets.
- Subject(s)
- Genre(s)
- ISBN
- 9781493991532 hardcover
1493991531 hardcover
9781493991549 ebook - Bibliography Note
- Includes bibliographical references and index.
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