Biophysical tools for biologists. Vol. 1, In vitro techniques / edited by John J. Correia, H. William Detrich, III
- Additional Titles:
- In vitro techniques
- Amsterdam : Boston : Elsevier, 
- Copyright Date:
- Physical Description:
- 1 online resource (xxiii, 972 pages, 12 unnumbered pages of plates) : illustrations
- Additional Creators:
- Correia, John J. (John Joseph) and Detrich, H. William, III
- Part I: History and Reflections -- Section 1. Solution Methods -- 1. Binding: A Polemic and Rough Guide -- Nichola C. Garbett, Jonathan B. Chaires -- 2. Linked Equilibria in Regulation of Transcription Initiation -- Dorothy Beckett -- 3. Biosensor-Surface Plasmon Resonance Methods for Quantitative Analysis of Biomolecular Interactions -- Farial A. Tanious, Binh Nguyen, and W. David Wilson -- 4. Isothermal Titration Calorimetry: Experimental Design, Data Analysis, and Probing Macromolecule/Ligand Binding and Kinetic Interactions -- Matthew W. Freyer and Edwin A. Lewis -- 5. Differential Scanning Calorimetry -- Charles H. Spink -- 6. Analytical Ultracentrifugation: A) Sedimentation Velocity, B) Sedimentation Equilibrium -- Tom Laue, Tom Moody, James Cole, and Jeff Lary -- 7. Determination of membrane protein molecular weights and association equilibrium constants using sedimentation equilibrium and sedimentation velocity -- Nancy K. Burgess, Ann Marie Stanley, and Karen G. Fleming -- 8. Basic Aspects of Absorption and Fluorescence Spectroscopy and Resonance Energy Transfer Methods -- Susan Bane and Natasha Shanker -- 9. Applications of Fluorescence Anisotropy to the Study of Protein-DNA Interactions -- Vince J. LiCata and Andy J. Wowor -- 10. Circular Dichroism and Its Application to the Study of Biomolecules -- Stephen R. Martin and Maria J. Schilstra -- 11. Folding and Stability -- Timothy O. Street, Naomi Courtemanche and Doug Barrick -- 12. Hydrodynamic shape modelling of analytical ultracentrifugation data -- Olwyn Byron -- 13. X-ray and neutron scattering data and their constrained molecular modelling -- Stephen J Perkins, Azubuike I. Okemefuna, Anira N. Fernando, Alexandra Bonner, Hannah E. Gilbert and Patricia B. Furtado -- 14. Structural Investigations into Microtubule-MAP Complexes -- Andreas Hoenger and Heinz Gross -- 15. Rapid Kinetic Techniques -- John F. Eccleston, Stephen Martin, and Maria J. Schilstra -- 16. Mutagenic Analysis of the Membrane Protein Functional Mechanisms: Bacteriorhodopsin as a Model Example -- George J. Turner -- 17. Quantifying DNA-protein interactions by single molecule stretching -- Mark C. Williams, Ioulia Rouzina, and Richard L. Karpel -- 18. Isotopomer-based metabolomic analysis by NMR and mass spectrometry -- Andrew N Lane, Teresa W-M. Fan, and Richard M. Higashi -- 19. Following molecular transitions with single residue spatial -- and millisecond time resolution -- Inna Shcherbakova, Somdeb Mitra, Robert Beer and Michael Brenowitz -- 20. Methods and applications of site-directed spin labeling EPR spectroscopy -- Candice S. Klug and Jimmy B. Feix -- 21. Fluorescence Correlation Spectroscopy and its application to the characterization of molecular properties and interactions -- Hac̈ne Boukari and Dan L. Sackett -- 22. Practical Guide to Osmolytes -- Jorg Rosgen and Daniel Harries -- 23. Stupid Statistics -- Joel Tellinghuisen -- 24. Nonlinear Least Squares Fitting Methods -- Michael L. Johnson -- 25. Methods for Simulating the Dynamics of Complex Biological Processes -- Maria J Schilstra, Stephen R. Martin, and Sarah M. Keating -- 26. Computational Methods for Biomolecular Electrostatics -- Feng Dong, Brett Olsen and Nathan A. Baker -- 27. Ligand effects on the protein ensemble: Unifying the descriptions of ligand binding, local conformational fluctuations, and protein stability -- Steven T Whitten, Bertrand Garc̕a-Moreno E., and Vincent J. Hilser -- 28. Molecular Modeling of Cytoskeletal Proteins -- Xiange Zheng and David Sept -- 29. Mathematical Modeling of Cell Motility -- Anders E. Carlsson and David Sept.
- Driven in part by the development of genomics, proteomics, and bioinformatics as new disciplines, there has been a tremendous resurgence of interest in physical methods to investigate macromolecular structure and function in the context of living cells. This volume in Methods in Cell Biology is devoted to biophysical techniques in vitro and their applications to cellular biology. The volume covers methods-oriented chapters on fundamental as well as cutting-edge techniques in molecular and cellular biophysics. This book is directed toward the broad audience of cell biologists, biophysicists, pharmacologists, and molecular biologists who employ classical and modern biophysical technologies or wish to expand their expertise to include such approaches. It will also interest the biomedical and biotechnology communities for biophysical characterization of drug formulations prior to FDA approval. * Describes techniques in the context of important biological problems * Delineates critical steps and potential pitfalls for each method * Includes full color plates to illustrate techniques.
- Bibliography Note:
- Includes bibliographical references and index.
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