Actions for Single domain antibodies [electronic resource] : methods and protocols

Email RIS file
Bookmark
Report an Issue

Single domain antibodies [electronic resource] : methods and protocols / edited by Dirk Saerens and Serge Muyldermans

Published
New York : Humana Press, c2012.
Physical Description
xv, 580 pages : illustrations (some col.) ; 27 cm.
Additional Creators
Saerens, Dirk and Muyldermans, Serge
Access Online

Availability

I Want It
I Want It

Finding items...

Series
Restrictions on Access
License restrictions may limit access.
Contents
From whole monoclonal antibodies to single domain antibodies : think small / Jean-Luc Teillaud -- Introduction to heavy chain antibodies and derived nanobodies / Cecile Vincke and Serge Muyldermans -- Overview and discovery of IgNARs and generation of VNARs / Stewart D. Nuttall -- Creation of the large and highly functional synthetic repertoire of human VH and Vk domain antibodies / Olga Ignatovich ... [et al.] -- Preparation of a naive library of camelid single domain antibodies / Aurelien Olichon and Ario de Marco -- Selection by phage display of single domain antibodies specific to antigens in their native conformation / Peter Verheesen and Toon Laeremans -- Semiautomated panning of naive camelidae libraries and selection of single-domain antibodies against peptide antigens / Jyothi Kumaran, C. Roger MacKenzie, and Mehdi Arbabi-Ghahroudi -- Pichia surface display : a tool for screening single domain antibodies / Kristof De Schutter and Nico Callewaert -- Bacterial two hybrid : a versatile one-step intracellular selection method / Mireille Pellis, Serge Muyldermans, and Cecile Vincke -- Intracellular antibody capture (IAC) methods for single domain antibodies / Tomoyuki Tanaka and Terence H. Rabbitts -- Selection of functional single domain antibody fragments for interfering with protein-protein interactions inside cells : a 'one plasmid' mammalian two-hybrid system / Tomoyuki Tanaka and Terence H. Rabbitts -- Cell-free selection of domain antibodies by in vitro compartmentalization / Armin Sepp and Andrew Griffiths -- Selection of VHHs under application conditions / Edward Dolk, Theo Verrips, and Hans de Haard -- Isolation and characterization of Clostridium difficile toxin-specific single-domain antibodies / Greg Hussack ... [et al.] -- Selection of VHH antibody fragments that recognize different Ab depositions using complex immune libraries / Rinse Klooster, Kim S. Rutgers, and Silvere M. van der Maarel -- Expression of single-domain antibodies in bacterial systems / Toya Nath Baral and Mehdi Arbabi-Ghahroudi -- Expression of VHHs in Saccharomyces cerevisiae / Andrea Gorlani, Hans de Haard, and Theo Verrips -- Stable expression of chimeric heavy chain antibodies in CHO cells / Vishal Agrawal ... [et al.] -- Production of Camel-Like Antibodies in Plants / Sylvie De Buck ... [et al.] -- Selecting and purifying autonomous human variable heavy (VH) domains / Raffi Tonikian and Sachdev S. Sidhu -- Solubility and stability engineering of human VH domains / Dae Young Kim, Wen Ding, and Jamshid Tanha -- Improvement of proteolytic stability through in silico engineering / Lucy Rutten, Hans de Haard, and Theo Verrips -- Selection of human VH single domains with improved biophysical properties by phage display / Kip Dudgeon ... [et al.] -- Improvement of single domain antibody stability by disulfide bond introduction / Yoshihisa Hagihara and Dirk Saerens -- Characterization of single-domain antibodies with an engineered disulfide bond / Greg Hussack, C. Roger MacKenzie, and Jamshid Tanha -- Affinity maturation of single-domain antibodies by yeast surface display / Akiko Koide and Shohei Koide -- Multivalent display of single-domain antibodies / Jianbing Zhang and C. Roger MacKenzie -- Methods for determining the PK parameters of AlbudAbs[TM] and of long serum half-life drugs made using the AlbudAb[TM] technology / Daniel Rycroft and Lucy J. Holt -- Fluorescent protein specific nanotraps to study protein-protein interactions and histone-tail peptide binding / Garwin Pichler, Heinrich Leonhardt, and Ulrich Rothbauer -- Site-specific labeling of his-tagged nanobodies with 99mTc : a practical guide / Catarina Xavier ... [et al.] -- Nanobody-based chromatin immunoprecipitation / Trong Nguyen Duc ... [et al.] -- User-friendly expression plasmids enable the fusion of VHHs to application-specific tags / Ario de Marco -- Application of single-domain antibodies in tumor histochemistry / Kien T. Maik and C. Roger MacKenzie -- Nanobodies as structural probes of protein misfolding and fibril formation / Erwin De Genst and Christopher M. Dobson -- Molecular imaging using nanobodies : a case study / Nick Devoogdt ... [et al.] -- Case study on live cell apoptosis-assay using lamin-chromobody cell-lines for high-content analysis / Kourosh Zolghadr ... [et al.].
Subject(s)
Genre(s)
ISBN
9781617799679 (hardcover : alk. paper)
161779967X (hardcover : alk. paper)
9781617799686 (eBook)
1617799688 (eBook)
Bibliography Note
Includes bibliographical references and index.
View MARC record | catkey: 32513863