Phosphorus-32 was used in tracer studies of the relationship between rhizosphere microorganisms and higher plants. Vigorous development of microorganisms within the rhizosphere was found to inhibit temporarily phosphorus entry inio oak and ash tree seedlings, but an appreciable portion of the phosphorus taken up by the microorganisms became available for the plants after 10 days. Rizosphere microorganisms were found to take up phosphorus compounds secreted by the seedling roots, thereby stimulating secretion of new portions of these compounds into the sand and water medium. A diurnal rhythm was noted both in the rate of root secretion of phosphorus and sulfur and in bacterial growth. Phosphorus containing root secretions also stimulated the growth of Azotobacter cells in the root system. (C.H.)