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A STUDY OF THE PENETRATION OF MAMMALIAN CELLS BY DEOXYRIBONUCLEIC ACIDS

Author
Borenfreund, E.
Published
United States : [publisher not identified], 1960.
[Oak Ridge, Tennessee] : [U.S. Atomic Energy Commission], 1960.
Physical Description
microopaque : positive ; 8 x 13 cm
Additional Creators
Bendich, A.

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Summary
S> Tritium-labeled deoxyribonucleic acid (DNA) from pneumococci and from human leukocytes was added to growing cultures of HeLa cells at 37C. Autoradiography revealed an extensive localization of H/sup 3/ in the nuclear regions. The label could not be removed by treatment with ribonuclease or dilute perchloric acid, but quantitative removal from the cells could be effected with deoxyribonuclease. Chemical and radioactivity determinations on nucleic acids isolated from the exposed HeLa cells revealed the presence of H/sup 3/ in all four DNA bases. About l2 mu g of tritiated DNA was recovered from 6 x 10/sup 6/ HeLa cells which had been exposed for 24 hr to 240 mu g of the human DNA. From this, it is concluded that the amount of DNA, or its degradation products, taken up by the cells was equivalent to at least 10 per cent of the normal HeLa cell complement. (auth)
Report Numbers
TID-6451
Other Subject(s)
Collection
U.S. Atomic Energy Commission depository collection.
Note
DOE contract number: AT(30-1)-910
NSA number: NSA-15-007145
OSTI Identifier 4104974
Research organization: Cornell Univ., New York. Medical Coll.
View MARC record | catkey: 42247641