Actions for Inferring inversion breakpoint evolution in homozygotes by examining chromatin architecture in Drosophila pseudoobscura

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Inferring inversion breakpoint evolution in homozygotes by examining chromatin architecture in Drosophila pseudoobscura

Author
Wright, Dynisty
Published
[University Park, Pennsylvania] : Pennsylvania State University, 2023.
Physical Description
1 electronic document
Additional Creators
Schaeffer, Stephen
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Open Access.
Summary
DNA is organized into chromosome territories, compartments, and topologically associated domains (TADs) within the nucleus. Chromosome rearrangements can alter this structure possibly leading to altered gene expression as well. The many mutations that can cause this are inversions, duplications, insertions, and more. In this thesis, we focus on inversion mutations. An inversion occurs when two breakpoints on one or two genes cause the sequence to orient in the opposite direction. In some species such as humans, inversions can cause negative effects; however, this does not occur in Drosophila. Instead, Drosophila harbors extensive inversion mutations, some of which are persistent in natural populations. Based on what we now know about chromatin architecture, we can understand more about the persisting inversions in Drosophila pseudoobscura. Chromatin architecture is vital throughout this thesis because we need to examine chromatin architecture to understand how TADs have evolved over a short-time scale. TADs are self-interacting domains that show preferential internal chromatin interactions with preferences for size, proteins, and structure involved in their formation. In D. pseudoobscura, seven different arrangements are separated by one persisting inversion: Standard (ST), Treeline (TL), Cuernavaca (CU), Pikes Peak (PP), Arrowhead (AR), Santa Cruz (SC), and Chiricahua (CH). We used TL and AR genomes to map TAD boundaries and breakpoints. We found that breakpoints are constrained to occur at boundaries possibly to prevent negative effects from occurring within D. pseudoobscura populations.
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Dissertation Note
Ph.D. Pennsylvania State University 2023.
Reproduction Note
Microfilm (positive). 1 reel ; 35 mm. (University Microfilms 30720652)
Technical Details
The full text of the dissertation is available as an Adobe Acrobat .pdf file ; Adobe Acrobat Reader required to view the file.
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