Targeted protein degradation / edited by George L. Burslem
- Published
- Cambridge : Academic Press, 2023.
- Copyright Date
- ©2023
- Physical Description
- 1 online resource (xvi, 323 pages) : illustrations (chiefly color).
- Additional Creators
- Burslem, George L.
Access Online
- Series
- Contents
- One - High-efficiency knock-in of degradable tags (dTAG) at endogenous loci in cell lines -- Two - High throughput E3 ligase degron binding assays for novel PROTAC ligand discovery -- Three - Synthesis of SNIPERs against BCR-ABL with kinase inhibitors and a method to evaluate their growth inhibitory activity derived from BCR-ABL degradation -- Four - Harnessing nanobodies for target protein degradation through the Affinity-directed PROtein Missile (AdPROM) system -- Five - Monitoring PROTAC interactions in biochemical assays using Lumit immunoassays -- Six - The In-Cell Western immunofluorescence assay to monitor PROTAC mediated protein degradation -- Seven - Cereblon target validation using a covalent inhibitor of neosubstrate recruitment -- Eight - Advancing targeted protein degrader discovery by measuring cereblon engagement in cells -- Nine - Quantitative measurement of PROTAC intracellular accumulation -- Ten - The importance of controls in targeted protein degradation: Determining mechanism -- Eleven - Crystallization of VHL-based PROTAC-induced ternary complexes -- Twelve - Targeted protein degradation through light-activated E3 ligase recruitment -- Thirteen - A heterobifunctional molecule system for targeted protein acetylation in cells
- Subject(s)
- ISBN
- 9780323992015 (electronic bk.)
0323992013 (electronic bk.)
9780323992008
0323992005 - Bibliography Note
- Includes bibliographical references.
View MARC record | catkey: 42253592