Actions for Direct and indirect immunofluorescence staining of fecal streptococci for rapid assessment of water quality

Email RIS file
Bookmark
Report an Issue

Direct and indirect immunofluorescence staining of fecal streptococci for rapid assessment of water quality

Author
Pavlova, M. T.
Published
United States : [publisher not identified], 1975.
[Oak Ridge, Tennessee] : [U.S. Atomic Energy Commission], 1975.
Physical Description
microfiche : negative ; 11 x 15 cm
Additional Creators
Beauvais, E., Brezenski, F. T., and Litsky, W.

Availability

I Want It
I Want It

Finding items...

Summary
Immunofluorescence (IF) techniques were employed in an attempt to develop a rapid test for the identification of fecal streptococci. Fresh isolates were obtained from river waters and raw sewage. Identification to species were made by the conventional physiological, biochemical, and serological tests. Both whole and disrupted cells of representative strains of each species were used for the preparation of the fecal streptococcal vaccine. Globulin fractions of individual and pooled antisera were labeled with fluorescein isothiocyanate, and the resulting conjugates were tested with homologous and heterologous antigens. The present findings suggest that the immunofluorescence techniques can be employed in the determination of the presence and source of fecal pollution in water employing the fecal streptococci as indicator organisms. By using this method it was determined that fecal streptococci can be identified from water and sewage samples within 20 hours. Parenthetically it should be noted that the identification procedures using the routine biochemical and serological tests may take as long as 7 to 14 days. The procedure may be automated for continual monitoring.
Report Numbers
BNL-23328; CONF-7511139-1
Other Subject(s)
Collection
U.S. Atomic Energy Commission depository collection.
Note
DOE contract number: EY-76-C-02-0016
OSTI Identifier 5214565
Research organization: Alabama Univ., Huntsville (USA).
Research organization: Brookhaven National Lab., Upton, NY (USA).
Research organization: Massachusetts Univ., Amherst (USA).
View MARC record | catkey: 42515253