Actions for Handbook of Human Oocyte Cryopreservation

Email RIS file
Bookmark
Report an Issue

Handbook of Human Oocyte Cryopreservation

Author
Porcu, Eleonora
Published
Cambridge : Cambridge University Press, 2012.
Physical Description
1 online resource (160 pages)
Additional Creators
Ciotti, Patrizia Maria and Venturoli, Stefano
Access Online

Availability

I Want It
I Want It

Finding items...

Contents
Chapter 1 Why freeze human eggs? Clinical and biological indications for human oocyte cryopreservation; Ethical and legal indications; Fertility-saving indications; IVF laboratory flexibility indication; The controversial family planning indication; Chapter 2 The evolution of human oocyte cryopreservation; Preliminary research; Early animal studies; Early human studies; Subsequent research; Recent clinical application; Safety of oocyte cryopreservation; Chapter 3 Clinical management of oocyte cryopreservation; Ovarian stimulation and oocyte storage., Storage of surplus eggs in a standard cycleEgg storage to avoid ovarian hyperstimulation syndrome; Egg storage in polycystic ovary syndrome; Egg storage in cancer patients; Chapter 4 Laboratory management of oocyte cryopreservation; The egg freezing laboratory: location, equipment, instruments, materials; Selecting freezable eggs: the freezable egg score; Evaluation of oocyte maturity and quality; Evaluation of cumulus-corona-oocyte complex maturity; Methodology; Evaluation of the nucleus; Evaluation of the cytoplasm; Cytoplasmic abnormalities; Extracytoplasmic abnormalities., Zona pellucida (ZP)Perivitelline space (PVS); Perivitelline space granules; First polar body fragmentation; Evaluation of the meiotic spindle; Grading of the oocyte; The freezable egg score; Survived oocyte; Degenerated first polar body; Degenerated oocyte; Oocyte with some sign of degeneration; Steps and timing of the entire cryopreservation procedure; Timing of the events that occur after HCG administration until embryo transfer; Timing of semen sample production; Organizing criteria for storage in liquid nitrogen tanks; Cryobiology; Chapter 5 Slow freezing; Principles., Evolution of the slow freezing methodologySlow freezing/rapid thawing; Materials; Solutions for slow freezing; Thawing solutions for human oocytes; Setting up for a freezing cycle; Setting up for a thawing cycle; Freezing solutions at different sucrose concentrations; Thawing solutions with differentiated sucrose concentrations; Setting up for a freezing cycle with differential sucrose concentrations; Setting up for a thawing cycle with differential sucrose concentrations; Recipe formula for Na depleted PBS., and Volume, temperature and time of exposure during slow freezing utilizing solutions with 0.1 or 0.2 or 0.3M sucrose at freezing and thawing (freezing 0.1M/thawing 0.1M-freezing 0.2M/thawing 0.2M-freezing 0.3M/thawing 0.3M)Volume, temperature and time of exposure during slow freezing utilizing solutions with differentiated sucrose concentrations (freezing 0.2M sucrose/thawing 0.3M); Starting the freezing cycle; Loading the straw into the programmable freezer; Beginning of the freezing program; Seeding; Storage of the samples; Automated computerized freezing machine; Rapid thawing; Methodology.
Summary
An indispensable practical tool for embryologists and reproductive medicine specialists seeking to offer their patients this revolutionary treatment option.
Subject(s)
Genre(s)
ISBN
9781139841955
1139841955
9781139839570 (electronic bk.)
1139839578 (electronic bk.)
9780511977879 (electronic bk.)
0511977875 (electronic bk.)
9780521192392
0521192390
Note
Volume, temperature and time of exposure during rapid thawing.
Bibliography Note
Includes bibliographical references and index.
View MARC record | catkey: 43172935