Adenosine triphosphate concentration in relation to microbial biomass in aquatic systems

Author: Cunningham, Jr, H. W.
Published: United States : [publisher not identified], 1977.
[Oak Ridge, Tennessee] : [U.S. Atomic Energy Commission], 1977.
Physical Description: microfiche : negative ; 11 x 15 cm

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Summary

Analyses of adenosine triphosphate (ATP) extracted from a sediment community of an aquatic ecosystem by the sulfuric acid method are complicated by inhibitions from inorganic and organic compounds. Inhibitions by inorganic compounds are reversible while those by organic compounds are irreversible. The primary inhibition by organic compounds results by complexing with acid-soluble fulvic acids which will prevent the detection of as much as 80% of the ATP present in a sample by the luciferin-luciferase reaction. Analytical techniques were developed to partially circumvent such interferences. Biomass interpretations from ATP concentrations in aquatic systems are complicated by the diversity of the microbiota and by the variability in the carbon to ATP ratio caused by environmental conditions. However, when levels of ATP are considered as a physiological condition of a sedimentary community, this data provides a means to interpret community metabolism not available hitherto.

Report Numbers

COO-1599-125(Pt.3)

Other Subject(s)

Collection

U.S. Atomic Energy Commission depository collection.

Note

DOE contract number: EY-76-S-02-1599
OSTI Identifier 7218601
Research organization: Michigan State Univ., East Lansing (USA). Dept. of Botany and Plant Pathology.

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