Actions for Synthesis of DNA containing uracil during bacteriophage infection of Bacillus subtilis. Technical progress report, August 1, 1975--July 31, 1976

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Synthesis of DNA containing uracil during bacteriophage infection of Bacillus subtilis. Technical progress report, August 1, 1975--July 31, 1976

Author
Price, A. R.
Published
United States : [publisher not identified], 1976.
[Oak Ridge, Tennessee] : [U.S. Atomic Energy Commission], 1976.
Physical Description
microfiche : negative ; 11 x 15 cm

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Summary
We have studied the synthesis of proteins and nucleic acids to understand how the bacteriophage PBS2 can make uracil-containing DNA in a cell which normally makes thymine-containing Bacillus subtilis DNA. We showed that the earliest known PBS2-induction of a protein is the inhibitor of the B. subtilis N-glycosidase (or uracil-DNA nuclease). This enzyme cleaves uracil from PBS2 DNA, and it is inhibited before any other known PBS2 protein appears in infected cells. The PBS2 dUMP kinase, which probably provides dUTP for uracil-DNA synthesis, has been partially characterized. Purification and induction in polymerase I- and III-deficient host cells indicate that the PBS2 DNA polymerase is a new enzyme. However, infection of temperature-sensitive polymerase III mutants suggests that the host's DNA polymerase III may play some role in PBS2 replication, at least under certain conditions. Two-dimensional thin-layer chromatography methods have been developed to quantitate the deoxyribonucleoside triphosphate pools before and after PBS2 infection.
Report Numbers
COO-2101-27
Other Subject(s)
Collection
U.S. Atomic Energy Commission depository collection.
Note
DOE contract number: E(11-1)-2101
OSTI Identifier 7234162
Research organization: Michigan Univ., Ann Arbor (USA). Dept. of Biological Chemistry.
Funding Information
Sponsored by US Energy Research and Development Administration (ERDA).
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