Actions for Synthesis of DNA containing uracil during bacteriophage infection of Bacillus subtilis. Technical progress report, August 1, 1976--October 31, 1977

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Synthesis of DNA containing uracil during bacteriophage infection of Bacillus subtilis. Technical progress report, August 1, 1976--October 31, 1977

Author
Price, A. R.
Published
United States : [publisher not identified], 1977.
[Oak Ridge, Tennessee] : [U.S. Atomic Energy Commission], 1977.
Physical Description
microfiche : negative ; 11 x 15 cm

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Summary
We have studied the biosynthesis of enzymes and nucleotides to understand how the bacteriophages PBS1 and PBS2 can make uracil-containing DNA in a cell which normally makes thymine-containing Bacillus subtilis DNA. Using our newly developed chromatographic system, we have discovered dUTP in infected cells (the first demonstration ever of this compound in vivo). Labelling studies in mutant cells proved that dUTP was derived from phage-induced dCTP deamination as well as ribonucleotide reduction. We also discovered two dTMP synthetases in B. subtilis, both of which remain active after PBS1 phage infection (although dTTP disappears from infected cells). The purified PBS2-induced DNA polymerase has been characterized physically and kinetically; it has similar affinities for uracil- and thymine-containing DNAs and triphosphates as substrates.
Report Numbers
COO-2101-32
Other Subject(s)
Collection
U.S. Atomic Energy Commission depository collection.
Note
DOE contract number: EY-76-S-02-2101
OSTI Identifier 5277076
Research organization: Michigan Univ., Ann Arbor (USA). Dept. of Biological Chemistry.
Funding Information
Sponsored by USDOE.
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