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Mutation induction in synchronous hamster cells

Author
Aebersold, P. M.
Published
United States : [publisher not identified], 1975.
[Oak Ridge, Tennessee] : [U.S. Atomic Energy Commission], 1975.
Physical Description
microfiche : negative ; 11 x 15 cm

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Summary
Mutagenesis of synchronous Mutahinese hamster cells by 5-bromodeoxyuridine (BUdR) shows pronounced cell cycle dependency. Resistance to 6-thioguanine (6-TG) and ouabain are induced maximally by BUdR at different times early in the DNA synthesis period, suggesting that the genes coding for hypoxanthine-guanine phosphoribosyltransferase (HGPRT) and the (Na+K+)-associated ATPase of the plasma membrane are replicated early in the DNA synthesis period. Although BUdR induces mutations in specific genes only when present during their replication, the rate of mutation induction is not linearly related to the amount of BUdR incorporated into DNA. The data show a BUdR concentration threshold for mutation induction, suggesting that BUdR exerts some deterimental allosteric effect on DNA synthesis enzymes.
Report Numbers
LBL-4720
Other Subject(s)
Collection
U.S. Atomic Energy Commission depository collection.
Note
DOE contract number: W-7405-ENG-48
OSTI Identifier 7364254
Research organization: California Univ., Berkeley (USA). Lawrence Berkeley Lab.
View MARC record | catkey: 45894539