Actions for Moessbauer spectroscopy of biochemicals containing iron. Progress report No. 7, October 1, 1969--September 30, 1973

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Moessbauer spectroscopy of biochemicals containing iron. Progress report No. 7, October 1, 1969--September 30, 1973

Author
May, L.
Published
United States : [publisher not identified], 1973.
[Oak Ridge, Tennessee] : [U.S. Atomic Energy Commission], 1973.
Physical Description
microfiche : negative ; 11 x 15 cm

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Summary
The purpose of this research is to utilize Mossbauer spectroscopy to study the structure of various hemoenzymes and hemoproteins and their relationship to biological activity. Initially, two hemoenzymes were selected: catalase and cytochrome c. To assist in the interpretation of the Mossbauer spectrum of cytochrome c, a study of the model compound, the undecapeptide, was made. This past year, cytochrome f was isolated from Euglena grown in a medium enriched with /sup 57/Fe. This protein is very similar to cytochrome c in its physical properties and is more easily enriched than cytochrome c. Another enzyme involved in drug and steroid oxidation, cytochrome P-450, is also being investigated. Also, a series of monomeric hemoglobins have been examined. These were selected because there had been studies made on the optical spectra and the electron spin resonance spectroscopy to which the Mossbauer parameters could add information concerning the state of the iron in these molecules. The Mossbauer spectrum of the bacteria, Hydrogenomonas Facilis, was also initiated to determine the usefulness of this technique in the study of the role of iron-containing proteins in this bacteria. The computer program for fitting the Mossbauer spectra has been translated into the Fortran IV language used by the PDP-10. (auth)
Report Numbers
ORO-4314-2
Other Subject(s)
Collection
U.S. Atomic Energy Commission depository collection.
Note
DOE contract number: AT(40-1)-4314
NSA number: NSA-29-007450
OSTI Identifier 4370213
Research organization: Catholic Univ. of America, Washington, D.C. (USA). Dept. of Chemistry.
View MARC record | catkey: 46176373